(JUNE 2012) 33. A plot of V/[S] versus V is generated for an enzyme catalyzed reaction, and a straight line is obtained. Indicate the information that can be obtained from […]
![(JUNE 2012) 33. A plot of V/[S] versus V is generated for an enzyme catalyzed reaction, and a straight line is obtained. Indicate the information that can be obtained from the plot. (1) Vmax and Km can be obtained only from a plot of 1/V versus 1/[S]. (2) Km/Vmax from the slope (3) Vmax and Km (4) only Km](https://www.letstalkacademy.com/wp-content/uploads/2025/09/33.jpg)
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CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Calculating Km and Ks in Michaelis-Menten Enzyme Kinetics: Strategy and Application
The correct answer is (4) Kₛ: 400 μM, Kₘ: 408 μM. Introduction Michaelis-Menten kinetics provides a foundation for understanding enzyme-catalyzed reactions. Two important constants—substrate dissociation constant (KsKs) and Michaelis constant (KmKm)—help […]
![(FEB 2022-11) 31. What is the fold difference between v at [S] =Km and v at [S] = 1000 Km where v is the initial velocity of an enzyme catalyzed reaction, [S] is substrate concentration and Km is the Michaelis constant? (1) 1.998 (2) 1000 (3) 2.998 (4) 3.998](https://www.letstalkacademy.com/wp-content/uploads/2025/09/31.jpg)
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Fold Difference in Initial Velocity Between Substrate Concentrations Km and 1000 Km in Michaelis-Menten Enzyme Kinetics
(FEB 2022-11) 31. What is the fold difference between v at [S] =Km and v at [S] = 1000 Km where v is the initial velocity of an enzyme catalyzed […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Determining Substrate Concentration Increase for Enzyme Reaction Rate Enhancement from 10% to 90% of VmaxVmax
(JUNE 2008) 30. For an enzyme catalyzed reactions exhibiting Michalis Menten equation what would be increase in substrate concentration to increase the rate of reaction from 10 % of Vmax […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Calculating Substrate Concentration for 90% Maximum Velocity in Michaelis-Menten Enzyme Kinetics
(DEC 2007) 29. In a biochemical reaction catalyzed by enzyme following the Michales-Menten equation, what will be the substrate concentration, when the observed velocity of reaction is 90% of the […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Calculating Initial Velocity Using Michaelis-Menten Kinetics: Alkaline Phosphatase Example
(FEB 2022-1) 28. The enzyme alkaline phosphatase was tested for its catalytic activity using the substrate para- nitrophenylphosphate. The KM obtained was 10 mM and Vmax was 100umol/min. Which one […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Substrate Concentration for Half-Maximal Velocity: Understanding Michaelis Constant (Km)
(JUNE 2010) 27. Km for enzyme is equal to where (1) V0=Vmax (2) 2V0= Vmax (3) 4V0=Vmax (4) V0 = 2 Vmax The correct answer is (2) Introduction In enzymatic reactions, […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Understanding Michaelis Constant (Km) and Its Relationship with Substrate Concentration and Initial Velocity in Enzyme Kinetics
(JUNE 2004) 26. During an enzyme assay if the initial velocity Vo = 1/2 Vmax, then substrate concentration will be equal to (1) α Km (2) Km (3) 1/2 Km […]
![(FEB 2022-1) 25. The following statements are being made to define the Michaelis constant (KM). It is: A. Independent of enzyme concentration [E] and substrate concentration [S] B. Equal to the dissociation constant when the [ES] complex dissociates more rapidly than product formation C. Equal to the dissociation constant when product formation is more rapid than [ES] complex dissociation D. An intrinsic property of an enzyme and does not depend on pH, temperature and ionic strength Which one of the following combination of statements is correct? (1) A and B only (2) A, B and D only (3) C and D only (4) A and D only](https://www.letstalkacademy.com/wp-content/uploads/2025/09/25.jpg)
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Michaelis Constant (Km) Explained: Key Properties and Assumptions in Enzyme Kinetics
(FEB 2022-1) 25. The following statements are being made to define the Michaelis constant (KM). It is: A. Independent of enzyme concentration [E] and substrate concentration [S] B. Equal to […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Understanding Enzyme-Substrate Affinity: The Role of Michaelis Constant (Km)
(JUNE 2007) 24. If the Km of enzyme for substrate A is 1 x 10-6 and for substrate B is 4 x 10-8, it means (1) Enzyme has more affinity […]
