Application of gene therapy in clinical trials did NOT succeed due to (1) poor integration of a gene in the host genome (2) lack of expression of integrated gene in […]
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CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
How to validate integrity of an introduced transgene in mice
Integrity of introduced transgene in mouse can be validated by (1) Male pronuclear insertion (2) Fusion of enucleated egg with somatic cells (3) Transfer into competent embryogenic cell (4) Southern […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Choosing the right expression system for glycosylated recombinant peptides
Which of the following system can be utilized for glycosylation of peptides expressed using recombinant DNA technology? (1) Large bacterial fermenters (2) Small bacterial fermenters (3) Normal bacterial bioreactors (4) […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Why the male pronucleus is used for transgene insertion in fertilized eggs
For making transgenic animals in fertilized egg the preferred place to insert trans-gene is in (1) Female pronuclei (2) Male pronuclei (3) Cytoplasm (4) Cleavage […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Selective lysis of newly dividing cells using BrdU and light
Which of the following methods can be used to selectively lyse newly dividing cells? (1) MTT (3-(4,5-Dimethylthiazol- 2-yl) – 2,5- diphenyltetrazolium bromide) treatment of dividing cells followed by UVB irradiation […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
How to selectively kill newly dividing mammalian cells in culture
If you want to selectively kill the newly dividing mammalian cells in a cell culture assay, which of the following methods will you use? (1) Exposure to UV radiation at […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Role of Leukemia Inhibitory Factor (LIF) in animal cell culture
Leukemia inhibiting factor has been utilized in animal cell culture for (1) Stimulating growth of cell (2) Inhibiting differentiation of stem cells (3) Inducing morphogenesis (4) Arrest cells at mitosis […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Matching genome-editing tools (ZFN, meganuclease, CRISPR–Cas9, TALEN)
Given below are terms related to Genome-editing tools in Column A and their feature in Column B. Which one of the following options is the most appropriate match between terms […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Hybrid nucleases in plant genome engineering
Which one of the following statements regarding use of hybrid nucleases for plant genome engineering is INCORRECT? (1) For gene knock out experiments, the nuclease is expressed without a donor […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Why EMS mutagenesis is used in TILLING
TILLING is a reverse genetics approach used in functional genomics. Which one of the following is used for TILLING? (1) T-DNA tagging by Agrobacterium-mediated transformation. (2) Transposon tagging using Ac/Ds […]
