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To prepare individual tissue cells from a primary culture, the cell-cell and cell-matrix interaction must be broken. To achieve this would NOT use: (1) EDTA            […]

Which one of the following statements is NOT correct for propagation and maintenance of mammalian cells in vitro? (1) Transformed cell lines do need external supply of serum to grow. […]

The following statements are made regarding developing a transgenic mouse: A. The transgenic mouse thus born will be a homozygous transgenic animal and can be maintained by crossing with another […]

14. Animal cell cultures are frequently used for production of therapeutic proteins. NIH3T3 (a fibroblast cell line) and CHO (Chinese hamster ovarian cell line) are some of the popular cell […]

13. Which one of the following techniques is generally used to produce transgenic animals? (1) Processed mRNA containing only exons are introduced into blastocyst stage embryo. (2) Entire foreign nucleus […]

To keep them in a totipotent state, embryonic stem cells need to be maintained in a medium supplemented with (1) growth hormone (2) leukemia inhibiting factor (3) nestin (4) insulin […]

For developing transgenic mice, embryonic stem cells are engineered to express the transgene. These cells are selected by (1) novobiocin. (2) neomycin. (3) tetracycline. (4) penicillin. The correct answer is (2) […]

The genes whose promoters are extensively used for production of pharmaceutical proteins in transgenic dairy cattles are (1) lactalbumin and ovalbumin. (2) lactoglobulin and casein. (3) lactoferrin and transferrin. (4) […]

Which of the following host systems is best suited to express large amounts of glycosylated protein for structural studies? (1) GST fusion protein in E. coli (2) His-tagged protein in […]

For sustainable expression of a trangene in the successive generation of a cell line in culture, the ideal gene transfer can be obtained using (1) lentiviral vector (2) adenoviral vector […]