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12. In the following diagram, segments A and C are copies of 10 base pair repeat DNA sequences, flanking a unique stretch shown as B, A and C are in […]

11. The following cassette was designed to create estrogen receptor knock-out mice: SoH: site of homology; Gol: gene of interest What would ensure that proper recombination has taken place? (1) […]

10. A student while constructing knock-out mice isolated mouse embryonic, stem cells and introduced an engineered DNA into the cells. However, none of the mice were transgenic. On checking the […]

9. In an effort to produce gene knockout mice, a gene targeted homologous recombination was tried, with the exogenous. DNA containing neor gene (confer G-418 resistance) and tkHsv gene (confers […]

8. To generate a knock-in or a knock-out mouse, it is essential to use antibiotic selection to select the stem cells that have putatively integrated the construct accurately. Each construct […]

7. Following statements have been made about recombination in a diploid organism: A. Recombination could be identified by genotyping parents and offspring’s for a pair of loci. B. Recombination frequency […]

6. During an experiment, an Investigator found that the cell line used are RecA -/-.                  What could be the probable finding which led him to such observation? (1) Low of […]

5. A Holliday junction js a branched nucleic acid structure that contains four double stranded arms joined together. After strand invasion and branch migration the function must then be resolved […]

4. To obtain recombinant products during meiosis, a double-strand break in the DNA yields crossovers needed for chiasmata formation. The progression of the non-crossover and crossover pathways begins with the […]

3.Column X lists proteins that play a role in mediating DNA recombination processes and Column Y lists the possible functions of these proteins. Column X Proteins Column Y Functions A. […]