1. E. coli cells were transformed with plasmid carrying tetracycline resistance gene and later on plated on LB medium and various colonies were observed but same number of colonies were […]
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CSIR NET Life Science Previous Year Questions and Solution on Genetics
Understanding Site-Specific Recombinases
21. Which of the following statements about site-specific recombinases is NOT true? (1) The Cre recombinase is believed to mediate the circularization of the Pl phage genome during infection of […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
Cre-LoxP System in Knockout Mice
20. Construction of knockout mice may be performed using the Cre-LoxP system. Eventually, the Cre recombinase of the bacteriophage P1 mediates site- specific recombination at a 34 bp sequence, lox […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
How LoxP Orientation Controls Inversion of the EFG Segment
19. Cre/loxP system is used by phage Pl to remove terminally redundant sequences that arise during packaging of the phage DNA. Cre-lox system can be used to create targeted deletions, […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
Enzymes Involved in Site-Specific Recombination
18. Site specific recombination results in precise DNA rearrangements, which is limited to specific sequences. The enzymes that are important to carry out the process are (1) Restriction endonuclease and […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
Cre‑Lox Strategy to Create Prostate‑Specific p53 Knockout Mice
17. In order to create an animal whose prostate gland is devoid of the p53 gene, engineered animals were created by cloning specially designed knock-out cassettes. Identify the most appropriate […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
Cre–loxP Mediated Exon Deletion
In a transgenic mice line, lox P sites are introduced in the target A in the following manner This transgenic mice line was mated with another transgenic mice line where […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
Tissue‑Specific p53 Knockout in Mouse Prostate Using Cre‑LoxP System
15. A student was asked to design a knockout cassette for specifically deleting the p53 gene from the prostate gland of mice. Which one of the following pairs of cassettes […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
Cre‑loxP System
14. In transgenic mice, the orientation an location of the loxP sites determine whether Cre recombinase induces a deletion, an inversion or a chromosomal translocation. If a researcher wants to […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
Understanding Cre-LoxP Site-Specific Recombination and loxP Site Orientation Effects
13. Which one of the following statements about Cre- mediated site-specific recombination at loxP sites is INCORRECT? (1) When loxP sites flanking a test sequence are oriented in same direction, […]
