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In a transduction experiment using genotype as a donor and a+b+c+ as a donor and a–b–c– as the recipient, a+ transductants were selected and screened for b and c. The […]

10. What is prophage (1) λ phage DNA (2) Stage of cell cycle (3) DNA of temperate phage inserted into host chromosome (4) A transposons Introduction Prophage represents a key […]

9. Transduction has been used extensively for genome mapping for bacteria. Which of the following process is useful for gene mapping? (1) Generalized transduction (2) Specialized transduction (3) Site specific […]

8. λ-phage insert their DNA into bacterial host. The site for recombination on host is termed as- (1) attp         (2) attB (3) Xis          (4) Int λ-phage inserts its DNA into […]

An experiment was designed to find out the relation between DNA uptake and transformation efficiency. 32P- labelled genomic DNA from Bacillus subtilis (A) and cold genomic DNA from Clostridium Jejeuni […]

6. In a transformation experiment, donor DNA from an E. coli strain with the genotypes was used to transform a strain of genotype Z+Y+. The frequencies of transformed classes were: […]

4. DNA was isolated from a strain of bacterium with genotype a+b+c+d+e+ and transformed into a bacterial strain a–b–c–d–e-. The transformants were tested for the presence of the donated genes. […]

5.  DNA from a bacterial strain with genotype at b+c+d+e+f+ was isolated and used to transform a strain of bacteria with genotype a–b–c–d–e–f–.The transformed cells were tested for the presence […]

DNA from a strain of bacteria with genotype a+b+c+d+e+ was isolated and used to transform a strain of bacteria that was a–b–c–d–e–. The transformed cells were tested for the presence […]

2. A lac– culture of E. coli was mutagenised. On what media would one spread the mutagenised cells to select for lac+ cells? (1) Minimal media+ lactose. (2) Rich media […]