Q71.Match List I with List II
| LIST I | LIST II |
|---|---|
| A. X-ray crystallography | I. Molecular weight determination |
| B. Mass spectrometry | II. Protein detection |
| C. Gel filtration | III. Protein structure |
| D. Western blotting | IV. Protein purification |
Choose the correct answer from the options given below:
(1) A-II, B-I, C-III, D-IV
(2) A-IV, B-III, C-II, D-I
(3) A-I, B-II, C-III, D-IV
(4) A-III, B-I, C-IV, D-II
X-ray crystallography determines protein 3D structures, while other techniques handle purification, mass, and detection. The correct answer is option (4): A-III, B-I, C-IV, D-II.
Match Analysis
A. X-ray crystallography → III. Protein structure. Correct—crystallizes proteins, diffracts X-rays to resolve atomic coordinates (PDB gold standard, 80%+ structures).
B. Mass spectrometry → I. Molecular weight determination. Correct—ionizes proteins, measures m/z ratio for exact mass (distinguishes isoforms/PTMs).
C. Gel filtration → IV. Protein purification. Correct—size-exclusion chromatography separates by hydrodynamic volume (larger elute first).
D. Western blotting → II. Protein detection. Correct—SDS-PAGE separates, transfers to membrane, probes with specific antibodies.
Option Breakdown
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(1) A-II, B-I, C-III, D-IV: Wrong—A ≠ detection; C ≠ structure.
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(2) A-IV, B-III, C-II, D-I: Wrong—A ≠ purification; B ≠ structure.
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(3) A-I, B-II, C-III, D-IV: Wrong—A ≠ mass; B ≠ detection.
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(4) A-III, B-I, C-IV, D-II: Perfect—each technique matched to primary function.
X-ray crystallography determines protein structure at atomic resolution, while mass spectrometry provides molecular weight precision for proteoforms.
Technique Applications Table
Workflow Integration
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Purify (gel filtration) → 2. Weigh (MS) → 3. Structure (X-ray) → 4. Detect (Western). Option (4) follows this logical pipeline.
Exam Strategy
CSIR/NEET mnemonic: “X Marks (structure) Mass (weight) Gel (purify) West (detect).” Visualize crystal → scale → column → blot.
