Q.40 Match the stationary phase (Column I) with its corresponding chromatography
technique (Column II).
Column I                                                                 Column II
P. Protein A                                                1. Size exclusion chromatography
Q. Sephadex                                                2. Ion-exchange chromatography
R. Phenylsepharose                                   3. Affinity chromatography
S. Diethylaminoethyl cellulose                4. Hydrophobic interaction chromatography
(A) P-1, Q-4, R-2, S-3
(B) P-3, Q-1, R-4, S-2
(C) P-3, Q-4, R-2, S-1
(D) P-4, Q-1, R-3, S-2

Master Chromatography Matching: Protein A, Sephadex, Phenylsepharose, DEAE-Cellulose Explained

Protein A serves as a ligand in affinity chromatography for purifying antibodies, while Sephadex enables size-based separations. The correct matching for these stationary phases with chromatography techniques is option (B).​

Correct Answer

Option (B) P-3, Q-1, R-4, S-2 matches each stationary phase accurately.​

Column I Breakdown

• P. Protein A: Binds specifically to Fc regions of IgG antibodies, defining affinity chromatography (3).​

• Q. Sephadex: Cross-linked dextran beads separate molecules by size in size exclusion chromatography (1).​

• R. Phenylsepharose: Phenyl groups on Sepharose promote hydrophobic interactions under high salt, suiting hydrophobic interaction chromatography (4).​

• S. Diethylaminoethyl cellulose: DEAE groups carry positive charge for anion exchange in ion-exchange chromatography (2).​

Options Analysis