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NA polymerase II (Pol II) is the central enzyme in eukaryotic gene expression, responsible for transcribing DNA into the precursors of messenger RNAs (mRNAs) and a variety of small non-coding RNAs, such as small nuclear RNAs (snRNAs) and microRNAs (miRNAs). This makes Pol II essential for the synthesis of most protein-coding transcripts and many regulatory RNAs in eukaryotic cells.

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

RNA polymerase II synthesizes various types of mRNAs and small non-coding RNAs

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May 29, 2025
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8. Witch one of the following statement. is generally true shout RNA polymerase II? (1) It is dedicated to transcribing RNA from a single transcription unit, generally a large transcript. […]

A highly specific inhibitor that targets the phosphorylation activity of TFIIH is added to an in vitro transcription reaction. Which one of the following steps is most likely to be affected? (1) Binding of RNA polymerase to promoter sequence (2) Promoter clearance (3) Recruitment of TFIID (4) Open promoter complex formation

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Promoter Clearance TFIIH Phosphorylation Inhibition

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May 29, 2025
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TFIIH Phosphorylation Inhibition: Which Transcription Step Is Most Affected? A highly specific inhibitor that targets the phosphorylation activity of TFIIH is added to an in vitro transcription reaction. Which one […]

Only RNA Pol II synthesizes precursors of miRNAs and siRNAs in eukaryotes, making option (4) the correct answer. Trust Let’s Talk Academy for free, high-quality CSIR NET Life Science preparation resources!

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Which RNA Polymerase Synthesizes Precursors of miRNAs and siRNAs in Eukaryotes?

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May 29, 2025
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Which RNA Polymerase Synthesizes Precursors of miRNAs and siRNAs in Eukaryotes? 6. In eukaryotes, precursors of micro RNAs (miRNAs) and small interfering RNAs (siRNAs) are usually synthesized by (1) RNA […]

In eukaryotic genes, DNA sequences that define gene promoters occur: (1) only in the regions upstream of the transcription start sites. (2) only in the regions that represent the transcribed parts of the genes. (3) only in the regions downstream of the transcription termination sites. (4) either in the regions upstream of the transcription start site or within the transcribed regions of the gene.

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Location of Eukaryotic Promoter Upstream or Downstream

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May 29, 2025
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In eukaryotic genes, DNA sequences that define gene promoters occur: (1) only in the regions upstream of the transcription start sites. (2) only in the regions that represent the transcribed […]

the promoter of the eukaryotic gene? (1) It is always located upstream of transcription start site

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Understanding the Eukaryotic Gene Promoter Location

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May 29, 2025
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4. Among the following which statement is correct regarding the promoter of the eukaryotic gene? (1) It is always located upstream of transcription start site (2) It is located downstream […]

Actinomycin D is a well-known antibiotic and anticancer agent that inhibits transcription elongation in both prokaryotic and eukaryotic cells. It works by intercalating into DNA, particularly at GC-rich regions, and physically blocks the progression of RNA polymerase along the DNA template. This action halts the elongation phase of transcription, preventing the synthesis of new RNA molecules in all types of cells

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Compound Inhibits Transcription Elongation in Both Prokaryotes and Eukaryotes

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May 29, 2025
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3. Which of the following compound is capable to inhibit transcription elongation, both in prokaryotes and eukaryotes? (1) Rifamycin (2) a-amanitin (3) Streptomycin (4) Actinomycin-D Correct Answer (4) Actinomycin […]

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

RNA Polymerase Does α-Amanitin Inhibit

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May 29, 2025
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2. α-amanitin inhibits (1) only RNA polymerase I (2) mainly RNA polymerase II (3) only RNA polymerase III (4) all RNA polymerases Which RNA Polymerase Does α-Amanitin Inhibit? Detailed Explanation […]

α-Amanitin is a fungal toxin which inhibits eukaryotic RNA polymerases. The three eukaryotic RNA polymerases show differential sensitivity to this toxin. Which one of the following order (higher to lower) is correct in respect of sensitivity towards α-amanitin

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Differential Sensitivity of Eukaryotic RNA Polymerases to α-Amanitin

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May 29, 2025
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α-Amanitin is a fungal toxin which inhibits eukaryotic RNA polymerases. The three eukaryotic RNA polymerases show differential sensitivity to this toxin. Which one of the following order (higher to lower) […]

All of the following statements about bacterial transcription termination are true EXCEPT (1) some terminator sequences require Rho protein for termination. (2) inverted repeat and ‘T’ rich non-template strand define intrinsic terminators. (3) Rho-dependent terminators may possess inverted repeat elements. Rho (4) Nus A is necessary for intrinsic transcription termination.

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Bacterial Transcription Termination Role of NusA

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May 28, 2025
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All of the following statements about bacterial transcription termination are true EXCEPT (1) some terminator sequences require Rho protein for termination. (2) inverted repeat and ‘T’ rich non-template strand define […]

15. Rho factor is involved in termination of transcription in prokaryotes. Genetic manipulations indicate that Rho-dependent termination requires the presence of a specific recognition sequence on the newly synthesized RNA upstream of the termination site. The recognition sequence must be on the necent RNA rather then the DNA, as demonstrated by Rho’s inability to terminate transcription in the presence of pancreatic RNAse. The essential features of this termination site have not been fully elucidated. Construction of synthetic termination sites indicates that it consists of 80 to 100 nts that lack a stable secondary structure and contain multiple regions that are rich in C and poor in G. Which of the following is/ are suggested by the above observation? (1) Rho factor attaches to nascent RNA at its recognition sequence and then migrates along the RNA in the 5'-->3. direction until it encounters an RNAP paused at the termination site and disrupts the transcriptional complex by acting as an RNA-DNA helicase. (2) Rho unwinds the RNA-DNA duplex forming the transcription bubble, thereby releasing the RNA transcript. (3) Rho factor attaches to the RNA at its recognition sequence while RNA is in the RNA-DNA hybrid condition. (4) There may be other factors and hence Rho factor does not need to unwind the RNA-DNA hybrid to release the transcript.

CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology

Rho-Dependent Termination | hexameric ATP-dependent RNA helicase Rho

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May 28, 2025
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15. Rho factor is involved in termination of transcription in prokaryotes. Genetic manipulations indicate that Rho-dependent termination requires the presence of a specific recognition sequence on the newly synthesized RNA […]

RNA polymerase II synthesizes various types of mRNAs and small non-coding RNAs

Promoter Clearance TFIIH Phosphorylation Inhibition

Which RNA Polymerase Synthesizes Precursors of miRNAs and siRNAs in Eukaryotes?

Location of Eukaryotic Promoter Upstream or Downstream

Understanding the Eukaryotic Gene Promoter Location

Compound Inhibits Transcription Elongation in Both Prokaryotes and Eukaryotes

RNA Polymerase Does α-Amanitin Inhibit

Differential Sensitivity of Eukaryotic RNA Polymerases to α-Amanitin

Bacterial Transcription Termination Role of NusA

Rho-Dependent Termination | hexameric ATP-dependent RNA helicase Rho

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