21.single -strand nick in parental DNA helix just ahead of a replication fork causesthe replication fork to break. Recovery from this calamity requires (1) DNA ligase (2) DNA primase (3) […]
Blog
Which Activity Is Absent in Bacterial DNA Polymerase I?
Among the following which activity is absent in bacterial DNA polymerase I (1) 5’-3’ Polymerase activity (2) 3’-5’ Polymerase activity (3) 5′-3’ Exonuclease activity (4) 3′-5′ Exonuclease activity Introduction […]
What Is the Proofreading Activity of DNA Polymerase? Exploring the Essential 3′→5′ Exonuclease Function
Among the following which iS termed as proof reading activity of DNA polymerase? (1) 5’à3′ polymerase activity (2) 3’à5’ polymerase activity (3) 5’à3′ exonuclease activity (4) 3’à5’ exonuclease activity […]
Error-Free Repair of DNA Double-Strand Breaks Is Accomplished by Homologous Recombination
Error-free repair of double strand breaks in DNA is accomplished by (1) non-homologous end-joining (2) base excision repair (3) homologous recombination (4) mismatch repair Error-Free Repair of DNA Double-Strand Breaks: […]
The Proofreading Mechanism of DNA Polymerase I in E. coli: Role of 3′→5′ Exonuclease Activity
Type of proofreading activity bv DNA polymerase I in E.coli is (1) 5’-3’ exonuclease (2) 5′-3′ endonuclease (3) 3′-5′ exonuclease (4) 3′-5′ endonuclease Introduction DNA Polymerase I (Pol I) […]
sos-repair-mechanism-involves-recombination-understanding-dna-damage-responses
Among the following which repair mechanism involves recombination? (1) Photo active direct repair mechanism (2) SOS repair mechanism (3) Post replication repair mechanism (4) Nucleotide excision repair mechanism Which DNA […]
Meiotic Recombination Does Not Involve Newly Replicated Identical DNA Duplexes: Key Features Explained
A meiotic recombination does not involve (1) formation of Holliday junction. (2) newly replicated identical DNA duplexes. (3) gene conversion. (4) crossover and non-crossover. Meiotic Recombination: What It Involves and […]
Predicting Agarose Gel Band Pattern After Repair of G:T Mismatch at HindIII Site in 6.4 Kb Plasmid Digested with HindIII and EcoRI
A 6.4 Kb plasmid DNA has two restriction endonuclease sites, HindIII and EcoRI. Complete double digestion of the plasmid with both the enzymes yields two fragments of 3.1 and 3.3 […]
DNA Damage Responses Explained: Damage Bypass, Reversal, and Removal with Key Repair Proteins
The complex responses to different types of DNAdamage in both prokaryotes and eukaryotes fall into three main categories: (i) damage bypass (ii) damage reversal (iii) damage removal Many repair proteins […]
Nucleotide Excision Repair in E. coli: Roles of Uvr Proteins, UvrD, and DNA Polymerase I Explained
Excision repair systems replace a short stretch of DNA around the site of damage. The following statement are made about nucleotide excision repair in E. coli: (A) UvrB homodimer creates […]


