Nick translation means (1) Translation by cytosolic ribosome (2) Translation of protein from stalled sites (3) Replication by ONA polymerase I after removing RNA primers (4) Replication of DNA by […]
Blog
Understanding Eukaryotic DNA Replication: Multiple Origins and Semi-Discontinuous Synthesis Explained
Which of the following statement is correct with reference to replication in eukaryotes? (1) Single origin and continuous replication (2) Multiple origin and semi-discontinuous replication (3) Multiple origin and continuous […]
Which DNA Polymerases Lack Proofreading Activity? Understanding 3′→5′ Exonuclease Functions in DNA Replication
Among the following which DNA polymerase lacks proof reading activity? (1) DNA polymerase α primase activity (2) DNA polymerase Ꟙ (3) DNA polymerase ϵ (4) Both DNA polymerase α and […]
Which DNA Polymerases Possess 3′→5′ Exonuclease (Proofreading) Activity in E. coli and Mammals?
DNA repair, synthesis and recombination are intimately connected and inter dependent. An apparent commonality between processes of DNA replication and repair in the enzymatically catalyzed synthesis of DNA polynucleotide segments, […]
Which Enzyme Removes RNA Primers During DNA Replication Using 5′→3′ Exonuclease Activity?
42, During replication, the RNA primer is degraded by the 5′ – 3′ exonuclease activity of (1) RNAseH (2) FEN-1 (flap endonuclease 1) (3) Topoisomerase II B (4) DNA polymerase […]
Understanding Leading Strand Synthesis and Ligase Deficiency Effects in Bidirectional DNA Replication
41. In the diagram below, the dotted line marks the point of initiation of bidirectional replication c, A. On the right side of the dotted line, leading strand synthesis occurs […]
Determining the Next Site of DNA Primase Action in Lagging Strand DNA Synthesis
40. The long DNA strand depicted below is serving as a template for lagging strand DNA synthesis. The short lines represent the newly synthesized okazaki fragments. At which position among […]
Bidirectional Replication and Okazaki Fragment Synthesis Order in Circular Bacterial Chromosomes
In the figure above, replication of DNA beginning from the origin of replication of the chromosome of a newly identified bacterium having a double stranded circular DNA genome is shown. […]
Decoding the Replication Fork: Which Okazaki Fragment Is Synthesized First?
Based on this information following statements are made: (i) represents the leading strand while (ii), (ii) and (iv) represent the Okazaki fragments. Among the Okazaki fragments, synthesis of (iv) occurs […]
Telomeres Are Made of Simple Tandem Repeats: Understanding Their Structure and Role in Chromosome Protection
Telomeres consist of (1) Simple tandem repeats (2) Nucleolar organizer region (3) GC rich sequences (4) Single copy repeats Introduction Telomeres are specialized structures located at the ends of […]


