Agrobacterium tumefaciens is frequently used as a vector to create transgenic plants. Under laboratory conditions Agrobacterium – mediated plant transformation does not require (1) host plant genes (2) bacterial type […]
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Interpreting T‑DNA hormone genes in Agrobacterium tumefaciens
The T-DNA region of the Ti plasmid of Agrobacterium tumefaciens harbors two genes: X and Y. Mutation of gene ‘X’ produces a rooty tumour while mutation of gene ‘Y’ produces […]
Which molecule activates Agrobacterium vir genes?
A set of virulence genes (vir genes), located in the Agrobacterium Ti-plasmid, is activated by (1) octopine (2) nopaline […]
Why acetosyringone is used in Agrobacterium‑mediated plant transformation
Treatment of acetosyringone is given during transfer of transgene using Agrobacterium as vector. The rationale behind this is that acetosyringone (1) Helps in anchorage of bacteria to plant cell wall […]
Bacillus thuringiensis as a biocontrol agent against insect pests
Which microbe has been used against insect plant pathogens? (1) Agrobacterium tumefaciens (2) Agrobacterium rhizogenes (3) Bacillus thuringenesis (4) Fusarium nudum Why Bacillus thuringiensis is correct Bacillus thuringiensis (Bt) is […]
Agrobacterium tumefaciens as the primary tool for generating transgenic plants
Which of the following organism have been extensively used in generation of transgenic plants? (1) Agrobacterium tumfaciens (2) Bacilus thuringensis (3) Baculo viruses […]
Which hormone delays Arabidopsis seed germination on MS medium?
Arabidopsis thaliana seeds were planted on Murashige Skoog (MS) plates with or without a hormone added to the medium. Seeds were found to germinate late in the hormone containing MS […]
Key facts about protoplast fusion in plant tissue culture
Protoplast fusion is used in plant tissue culture for various applications. In protoplast fusion: A. naked plant cells are used. B. transfer of organelles is not possible. C. partial genome […]
Somatic hybridization
Following are certain statements regarding somatic hybridization, a technique used for plant improvement. A. Protoplasts of only sexually compatible plant species can be fused. B. Hybrids are produced with variable […]
Key concepts in plant tissue culture
The following statements are related to plant tissue culture A. Friable callus provides the inoculum to form cell- suspension cultures. B. The process known as ‘habituation’ refers to the property […]


