To increase the shelf-life of tomato, which of the following genes should be silenced? (1) ACC synthase (2) EPSP synthase (3) […]
Blog
Which Agrobacterium Vir proteins act inside plant cells?
Agrobacterium Ti plasmid vectors are used to generate transgenic plants. The following are examples of vir gene encoded proteins that are important for transfer of T-DNA in to plants. A- […]
Functions of Vir proteins during Agrobacterium T-DNA transfer
Match the following Vir proteins with their correct function during Agrobacterium mediated transfer of T- DNA to plant cells. (1) A-(i), B-(ii), C-(iii), D-(iv) (2) A-(iv). B-(i). C-(ii), D-(iii) (3) […]
Sequence of events in Agrobacterium T-DNA transfer
Given below are four statements related to Agrobacterium-mediated transfer of T-DNA into plant cells: A. Production of single-stranded T-DNA by VirD1 and VirD2 proteins. B. Interaction of VirE2 with VIP1 […]
Why Agrobacterium-based transgenic plants do not form tumors
Agrobacterium tumefaciens, also known as natural genetic engineers, causes crown-gall disease in plants. However, when the same bacteria are used to raise transgenic plants with improved agronomic traits, no such […]
Key genetic elements in Agrobacterium-mediated plant transformation
Following are some statements related to raising transgenic plants A. T-DNA of Agrobacterium contains oncogenes and opine synthesis genes B. All 8 operons present in vir region of Agrobacterium is […]
Key facts for designing Agrobacterium-mediated plant transformation experiments
While designing an experiment for Agrobacterium mediated plant transformation, a student noted down the following points: A. Ti and Ri plasmids induce crown gall and hairy root disease, respectively B. […]
Understanding Ti plasmid regions and Agrobacterium diseases in plant transformation
Following are certain statements regarding the use of Agrobacterium in plant transformation. A. A. tumefaciens causes crown gall disease and A. rhizogenes causes hairy root disease. B. Region A in […]
Key facts about Agrobacterium tumefaciens, opines and crown gall callus
A student noted the following points regarding Agrobacterium tumefaciens: A. A. tumefaciens is a gram-negative soil bacterium. B. Opine catabolism genes are present in T- DNA region of Ti-plasmid. C. […]
Opines and Agrobacterium virulence
Which one of the following statements is correct? (1) None of the virulence genes of Agrobacterium tumefaciens are expressed constitutively. (2) Integration of T-DNA with the nuclear genome of plant […]


