β-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume 100 liters and a voidage (ε) […]
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Residence Time Calculation
β-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume 100 liters and a voidage (ε) […]
Is the lac Operon Repressed or Induced?
An E. coli cell of volume 10−12 cm3 contains 60 molecules of lac-repressor. The repressor has a binding affinity (Kd) of 10−8 M and 10−9 M with and without lactose […]
Molar Concentration of Lac Repressor
An E. coli cell of volume 10−12 cm3 contains 60 molecules of lac-repressor. The repressor has a binding affinity (Kd) of 10−8 M and 10−9 M with and without lactose […]
Enzyme Purification Yield Calculation
The purification data for an enzyme is given below: Step Process Volume (ml) Total protein (mg) Total activity (Units) Specific activity (Units/mg) P Cell-free extract 17 177 102 0.58 Q […]
Fold Purification Calculation
The purification data for an enzyme is given below: Step Process Volume (ml) Total protein (mg) Total activity (Units) Specific activity (Units/mg) P Cell-free extract 17 177 102 0.58 Q […]
Free Energy Change for K⁺ Ion Transport in Muscle Cells
In a muscle, the extracellular and intracellular concentrations of Na+ are 150 mM and 12 mM, and those of K+ are 2.7 mM and 140 mM, respectively. Assume that the […]
Free Energy Change
In a muscle, the extracellular and intracellular concentrations of Na+ are 150 mM and 12 mM, and those of K+ are 2.7 mM and 140 mM, respectively. Assume that the […]
Unraveling Antibiotic Action
Q.47 A bacterial culture (200 μl containing 1.8 × 109 cells) was treated with an antibiotic Z (50 μg per ml) for 4 h at 37°C. After this treatment, the […]
Substrate Inhibition Kinetics
Q.46 An enzymatic reaction is described by the following rate expression: v = vmS km + S + S2 / ks Which one of the following curves represents this […]


