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26. High performance liquid chromatography (HPLC) cannot be used to: A. Identify the various pigments from a leaf extract B. Separate organic pesticides C. Determine the caffeine content in coffee […]

25. In gas chromatography, the concentration of a substance can be determined by: A. Comparison of the area under the peak produced by the substance with the areas under the […]

24. Which of the following statements about chromatography is correct? A. Paper chromatography and gas chromatography are both routinely used for qualitative analysis only. B. Paper chromatography is usually considered […]

23. In reverse phase chromatography, the nature of the stationary phase is: A. Non-polar B. Polar C. Either non-polar or polar D. None of these Reverse phase chromatography uses a […]

22. The purity of a solute collected between two times t1 and t2 during chromatographic separation is: A. Amount of solute eluted − amount of impurity eluted B. Amount of […]

21. Chromatography is based on the: A. Different rate of movement of the solute in a column B. Separation of one solute from other constituents by being captured on the […]

20. In a gel filtration column: A. Smaller proteins enter the beads more readily B. Larger proteins elute first C. Both A and B D. Large proteins enter the beads […]

19. Which one of the following techniques is suitable for the large-scale purification of isozymes (A and B) that differ from each other by a single positively charged amino acid? […]

18. Which one of the following is true during the separation of biomolecules by reversed-phase chromatography? A. Stationary phase is less polar than the mobile phase B. Stationary phase is […]

17. In Sephadex G series (gel filtration), as the G number increases from 10, 15, 25 … 200, the pore size of the beads: A. Decreases B. Increases C. Remains […]