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46. Chromatography involves two mutually: A. Immiscible phases B. Miscible phases C. Soluble phases D. None of the above Correct Answer: A. Immiscible phases Chromatography requires two phases that do […]

45. Chromatography is the process for identification, purification and separation of components of a mixture on the basis of: A. Difference in their boiling point B. Difference in their melting […]

44. In reversed-phase HPLC: A. A hydrophilic stationary phase is combined with a non-polar mobile phase B. A hydrophobic stationary phase is combined with a polar mobile phase C. A […]

43. In anion exchange chromatography, A. The column contains negatively charged beads where positively charged proteins bind B. The column contains positively charged beads where negatively charged proteins bind C. […]

42. The use of insulin hormone to purify its receptor is an example of (A) Ion exchange chromatography (B) Affinity chromatography (C) Gel filtration chromatography (D) Ligand mediated chromatography Answer: […]

41. In gel filtration chromatography, separation of proteins are based on their (A) Size and net charge (B) Size and shape (C) Size and specific affinity (D) Shape and net […]

40. In which of the following separation method where proteins are separated on the basis of their net charge (A) Affinity chromatography (B) Ion exchange chromatography (C) Dialysis (D) Gel […]

39. Which of the following statements is incorrect? A. In affinity chromatography, lectins are used to purify a glycoprotein B. The separation in gel filtration chromatography is based on size, […]

38. In a mixture of the five proteins listed below, which should elute second in size-exclusion (gel-filtration) chromatography? A. Cytochrome c (Mw = 13,000) B. Immunoglobulin G (Mw = 145,000) […]

37. Which of the following methods could be used to check the molecular weight of your purified protein? A. SDS-PAGE only B. Mass spectrometry only C. Analytical size exclusion chromatography […]