Proteins A and B having a molecular weight of 16500 and 35400 move 1.3 cm and 4.6 cm, respectively, when electrophoresed through a gel. What is the molecular weight of […]
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CSIR NET Previous Year Questions Gel Electrophoresis
The relative electrophoretic mobilities of a 30-kd protein and a 92-kd protein used as standards on an SDS
The relative electrophoretic mobilities of a 30-kd protein and a 92-kd protein used as standards on an SDS polyacrylamide gel are 0.80 and 0.41, respectively. What is the apparent mass […]
CSIR NET Previous Year Questions Gel Electrophoresis
What is an issue with using 2D-PAGE
What is an issue with using 2D-PAGE? a. Hydrophobic proteins may not run as expected due to the hydrophobic surfaces. b. Some proteins may not migrate through polyacrylamide and therefore […]
CSIR NET Previous Year Questions Gel Electrophoresis
SDS Used in the Electrophoresis of Proteins
Why is SDS used in the electrophoresis of proteins? a. SDS coats the protein with a negative charge so that the sample can run through the gel. b. SDS is […]
CSIR NET Previous Year Questions Gel Electrophoresis
Important for Gel Electrophoresis to work
Which of the following is important for gel electrophoresis to work? a. Negatively charged nucleic acids to migrate through the gel. b. Ethidium bromide to provide a means to visualize […]
CSIR NET Previous Year Questions Gel Electrophoresis
Prepare RNA for Northern blot analysis
A researcher needs to prepare RNA for Northern blot analysis. Initial experiments using total RNA produce no signal when the experiment is completed. A method to increase the sensitivity of […]
CSIR NET Previous Year Questions Gel Electrophoresis
Isoelectric focusing separation of protein-on positive and negative
What is the basis of isoelectric focusing separation of protein. (a) Relative content of positively charged residues only (b) Relative content of negatively charged residues only (c) Size (d) Relative […]
CSIR NET Previous Year Questions Gel Electrophoresis
Approaches is used to measure the approximate mass of the purified protein complex and to determine its subunit composition
Which of the following approaches is used to measure the approximate mass of the purified protein complex and to determine its subunit composition? (a) Polyacrylamide gel electrophoresis and molecular cloning […]
CSIR NET Previous Year Questions Gel Electrophoresis
Technique that can be used to analyze proteins-isoelectric focusing?
The correct statement for a technique that can be used to analyze proteins. Which of the following is true of isoelectric focusing? (a) It is commonly used to quantitate IgA […]
CSIR NET Previous Year Questions Gel Electrophoresis
Fragment of DNA from EtBr stained gel
How can we pick a fragment of DNA from EtBr stained gel, How would you pick the fragment you are interested in? (a) By digesting with an enzyme, which will […]
