The correct statement for a technique that can be used to analyze proteins. Which of the following is true of
isoelectric focusing?
(a) It is commonly used to quantitate IgA
(b) It separates proteins in an aqueous environment where one is able to maintain a net charge of +1 on the proteins
(c) It is restricted to the analysis only in an acidic environment (pH < q)
(d) It separates proteins based on the pH at which the net charge on a protein is zero
The correct answer is:
(d) It separates proteins based on the pH at which the net charge on a protein is zero.
Explanation:
- Isoelectric focusing (IEF) is a technique used to separate proteins based on their isoelectric point (pI), which is the pH at which a protein carries no net charge.
- This is achieved by running proteins through a pH gradient in a gel (usually polyacrylamide or agarose) under an electric field.
- Proteins migrate through the gradient until they reach their pI, where they stop moving because they are neutral (no net charge).
Why Not the Other Options?
- (a) It is commonly used to quantitate IgA → IEF is not used for quantifying immunoglobulins; techniques like ELISA or nephelometry are better suited for that.
- (b) It separates proteins in an aqueous environment where one is able to maintain a net charge of +1 on the proteins → Incorrect because proteins do not all have a net charge of +1; they migrate based on their individual pI.
- (c) It is restricted to the analysis only in an acidic environment (pH < q) → Incorrect because IEF uses a wide pH range (acidic to basic) to separate proteins based on their pI, not just in an acidic environment.
Thus, option (d) is correct because IEF separates proteins based on their isoelectric point (pI), where they have no net charge.
75 Comments
Prami Masih
February 27, 2025Super sir
Manisha choudhary
August 16, 2025Done sir
Isoelectric focusing wide ph range p hoti h (acidic to basic)
Jaha protein ko pi value k base p mtlb at which ph jaha protein p net charge zero ho jaaye
Mahima Sharma
August 24, 2025Seperation of protein based on the ph at which net charge of protein is 0
Arushi
April 1, 2025👍✔️
Mahima Sharma
August 15, 2025Ph at which net charge is 0
Anita choudhary
April 18, 2025Done sir 👍
Priya khandal
August 13, 2025Ok sir
Mohd juber Ali
August 14, 2025Option (d) is right isoelectric focusing seprate protein on the basis of PI isoelectric point or charge
pH at which net charge is zero= PI
shruti sharma
August 14, 2025option d is correct
Khushi Agarwal
August 14, 2025Correct answer is 4
It separates proteins based on the pH at which the net charge on a protein is zero
Dp
August 14, 2025IEF-net charge zero
Anurag Giri
August 14, 2025Isoelectric focusing (IEF) is a technique used to separate proteins based on their isoelectric point (pI), which is the pH at which a protein carries net charge zero
Priya dhakad
August 14, 2025Isoelectric focusing separates proteins based on the pH at which the net charge on a protein is zero.
Aman Choudhary
August 14, 2025option d is correct
IEF separate the protein based on PI at which net charge is zero
Neelam Sharma
August 14, 2025It separates proteins based on the pH at which the net charge on a protein is zero.
Niti Tanwar
August 14, 2025Isoelectric focusing separate protein based on pH at which tha net charge on a protein is 0.
Mansukh Kapoor
August 14, 2025The correct answer is option 4th because IEF is the technique which separates the protein on the basis of pH having the net charge zero
Soniya Shekhawat
August 14, 2025IEF separate protein all the basis pH where net charge of protein is zero.
Monika meena
August 27, 2025IEf separate the protein on the basis of iso electric point at which they become net Charge is zero on protein
Urmi sisodiya
August 14, 2025Correct answer is d bcoz in ief net charge on protein is zero and it seperate protein based on ph
Urmi sisodiya
August 14, 2025bcoz in ief net charge on protein is zero and it seperate protein based on ph
Aafreen
August 14, 2025Option 4 is correct
It separates proteins based on the pH at which the net charge on a protein is zero
Anjani sharma
August 14, 2025Answer 4 as the proteins are separated on the basis of charge at which net charge is zero – IEF
Pratibha
August 14, 2025Correct answer is 4
IEF separates proteins based on the pH at which the net charge on a protein is zero
Sakshi vijay
August 14, 2025Correct answer is 4
Because IEF seperate protein on ph bases where net charge on protein is zero
Manisha gujar
August 14, 2025Option d is correct
Dipti Sharma
August 14, 2025proteins separated based on the pH at which the net charge on a protein is zero is IEF
Karishma
August 14, 2025Isoelectric focusing separates protein on the basis of ph at which the net charge on a prtotein is 0
Priti khandal
August 14, 2025option day is correct sir it separate protein based on the pH at which the net charge on a protein is zero
Priya khandal
August 14, 2025It’s separate protein based on the pH at which the net charge on a protein zero done sir
Deepika Sheoran
August 15, 2025Protein saprated based on the PH at which the net charge on a protein is zero is isoelectric focusing.
Santosh Saini
August 15, 2025It separate protein based on the pH at which the net charge is zero=PI
Priyanka choudhary
August 16, 2025Option d is correct answer,because pH at which net charge is zero
Samiksha bajiya
August 17, 2025Separation of protein on the basis of ISO electric point or charge
Mahima Sharma
August 16, 2025Seperation of protein based on ph which net charge is 0
Nilofar Khan
August 16, 2025Correct answer is d
Divya rani
August 17, 2025D is right because IEF is a technique which separate the protein on the basis of pi at zero
Kavita choudhary
August 17, 2025Answer d is correct because separation of protein is based apon ph is net charg zero
Sneha .......
August 20, 2025IEf separate proteins charge based on ph
Surbhi Rajawat
August 17, 2025Answer is d. IEF separates the protein on the basis of their isoelectric point that is the Ph at which they become neutral. IEF is a part of 2D gel.
Parul Yadav
August 17, 2025IEF separates proteins on the basis of their specific pH at which there is no net charge on them i.e. their isoelectric point.
Monika meena
August 27, 2025IEf separate the protein on the basis of iso electric point that is ph at which they become net Charge is zero on protein
Avni
August 17, 2025The correct answer is that it separates proteins based on the pH at which the net charge on a protein is zero.
Kajal
August 17, 2025It separate the protein on the basis of ph where the net charge on the protein is zero
Shrddha Modi
August 17, 2025Pi where net charge is zero Option d
Bhavana Kankhedia
August 24, 2025it separates proteins based on the pH at which the net charge on a protein is zero.
Bharti Yadav
August 24, 2025It separates proteins based on the pH at which the net charge on a protein is zero.
Payal Gaur
August 24, 2025It’s seprate protein based on the pH at which net charge on protein is zero
Priyanka Choudhary
August 24, 2025D is right answer,separates proteins based on the pH at which the net charge on a protein is zero.
Sonam
August 24, 2025Correct answer is 4
Alec
August 24, 2025option d – it separates the protein based on the ph at which net charge on protein is zero.
Anjali
August 24, 2025separates proteins based on the pH at where the net charge on a protein is zero.
Falguni Prakash
August 24, 2025Option D
Sakshi
August 24, 2025It’s separate protein based on pH at which net charge of protein is zero
Vijay kumar Meena
August 24, 20254 means d correct
Ayush Dubey
August 25, 2025It separates proteins based on the pH at which the net charge on a protein is zero
Devika
August 25, 2025It separates protein based on ph at which the net charge on protein is zero
Kashish yadav
August 25, 2025Option d
sakshi khatri
August 25, 2025d is correct
sakshi khatri
August 25, 2025done
Shivani Panwar
August 25, 2025Correct answer is 4
Harish Kumar
August 26, 2025Correct answer because protein separate on PH
Surendra Doodi
August 27, 2025??
Pratibha Jain
August 30, 2025Correct answer is option (d)
IEC separates proteins based on the pH at which the net charge on a protein is zero
Kalpana
August 30, 2025Option D
Sheikh Aleen
August 30, 2025It separates proteins based on the pH at which the net charge on a protein is zero
Anisha Beniwal
August 30, 2025separates proteins based on the pH at which the net charge on a protein is zero.
Akshita Jangid
August 31, 2025Option D It separates proteins based on the pH at which the net charge on a protein is zero.
Shobha Kanwar
August 31, 2025Seperation of protein based on the ph at which net charge of protein is 0
Asha Gurzzar
August 31, 2025Option d is correct as isoelectric focusing seperate proteins based on ph at which net charge on protein is 0
Anisha jakhar
September 3, 2025(d) It separates proteins based on the pH at which the net charge on a protein is zero.
Muskan Yadav
September 3, 2025It separates proteins based on the pH at which the net charge on a protein is zero.
Somya Yadav
September 5, 2025It separates proteins based on the pH at which the net charge on a protein is zero.
Aakansha sharma Sharma
September 29, 2025The correct answer is:
(d) It separates proteins based on the pH at which the net charge on a protein is zero.
Vanshika Sharma
January 16, 2026It separates proteina based on the ph at which the net charge is zero