Proteins are commonly purified by ion exchange chromatography (IEC) as a final step. Which of the following statements is NOT true? 1. above the isoelectric point, the proteins bind to […]
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Understanding the Need for Sensitivity in Assay Method Modifications
33. You have an assay method that can estimate compound A upto level 10 mg/ml. If you need to modify it so that you can estimate 0.1 mg/ml, you need […]
Role of Endothecium in Anther Dehiscence: Structure and Function
The mature anther wall comprises an epidermis followed by a layer of radially elongated cells with fibrous bands of callose called endothecium whose function is 1. mechanical 2. protection 3. […]
Exploring Techniques for Studying Protein-Peptide and Protein-DNA Interactions
68. Which one of the following techniques can be utilized to study both protein–peptide and protein–DNA interactions? (1) DNA footprinting (2) 2D-gel electrophoresis (3) Phage display (4) ChIP-on-chip assay 🧬 […]
Understanding the Chip-on-Chip Technique: Identifying Transcription Factor Binding Regions
32. Chip-on-chip, a technique that combines chromatin immune precipitation with microarrays, is used to identify: (a) micro-RNA coding genes, (b) Protein motifs involved in protein-protein interaction, (c) Protein-coding regions in […]
What Are Lectins? The Key Probes for Analyzing Glycoproteins
The probe used to analyse glycoproteins is 1. interferons 2. cytokine 3. lectins 4. glutens Understanding Glycoproteins and Their Analysis Glycoproteins are proteins that contain carbohydrate groups attached to […]
Identifying Fake Medicines: How to Detect Pharmaceutical Counterfeits
31. Fake medicines are a nuisance that shatter the faith of patients in medicine and enhance the emergence of drug resistance. A medicine is likely to be fake if: (a) […]
67. Following statements are about chromatin organisation in eukaryotes: i. The length of DNA per nucleosome varies for individual tissue or species. ii. Typical nucleosomal packaging pattern is strictly maintained […]
Understanding Gel Filtration Chromatography: Separation Based on Hydrodynamic Volume
30. Gel filtration chromatography separates proteins on the basis of: (a) Affinity tag, (b) Charge, (c) Hydrodynamic volume, (d) Hydrophobicity Introduction Gel filtration chromatography, also known as size exclusion chromatography […]
What Happens When S Phase and G2 Phase Mammalian Cells Are Fused? Exploring Cell Cycle Regulation
66. Which one of the following result is expected when a mammalian cell in S phase is fused with another in G2? (1) G2 phase nucleus will wait for the […]


