123. Which one of the following will NOT change upon adding an enzyme to a chemical reaction or changing the concentration of reactants or products? A. βπΊΒ° B. πΎππ C. […]
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Title: Understanding Recombination Signal Sequences in V(D)J Recombination
52. Which one of the following is NOT a part of the recombination signal sequence in VDJ recombination? 1. A conserved 7 bp consensus sequence 2. Locus control regions (LCR) […]
Cell Cycle-Based Cell Separation: The Power of Counterflow Centrifugal Elutriation
122. Which one of the following cell separation techniques allows cells from different stages of the cell cycle to be separated simultaneously with minimal perturbation to their physiology? A. counterflow […]
Impact of Folic Acid Deficiency on Amino Acid Biosynthesis Pathways
51. Which one of the following amino acid biosynthesis pathways is affected in folic acid deficiency? 1. Aspartate from oxaloacetate & glutamate 2. Glycine from glucose and alanine 3. Glutamate […]
Title: Polyadenylation Modifications in Eukaryotic Pre-mRNA: Key Positions
50. In an eukaryotic pre-mRNA the number of positions where polyadenylate modifications occur is: 1. 5 2. 2 3. 3 4. 4 PART B Polyadenylation Modifications in Eukaryotic Pre-mRNA: An […]
Role of 2′, 3′ Dideoxy-ribonucleoside Triphosphates in DNA Sequencing
121. Which one of the molecular processes utilize 2β², 3β² Dideoxy-ribonucleoside triphosphates? A. DNA synthesis for primers. B. Okazaki fragment formation during lagging strand synthesis. C. DNA sequencing by synthesis. […]
Taq DNA Polymerase vs. Klenow Fragment: Key Differences in Enzyme Activity
48. Taq DNA polymerase differs from the Klenow fragment in having: 1. 5β²-3β² polymerase activity 2. 5β²-3β² exonuclease activity 3. 3β²-5β² exonuclease activity 4. Endonuclease activity Taq DNA Polymerase vs. […]
Glycosylation of Proteins in the ER: Where Do Glycan Moieties End Up?
120. Proteins are glycosylated in the endoplasmic reticulum. In the final structures, the glycan moieties are typically present: A. on the surface of these proteins. B. in the core of […]
Understanding Nick Translation Protocol: Effect of Gamma Phosphate-Labeled ATP in DNA Labeling
47. Gamma phosphate labelled ATP molecule was used to label a DNA fragment using the nick translation protocol. This would result in: 1. Labelling at 5β²end 2. Labelling at 3β²end […]
Understanding PCR Primer Design: Identifying the Reverse Primer for DNA Amplification
46. A DNA with the sequence 5β²CGCATCGATCATGCCCTGAβ¦..AGTCCCATTAGATGCC3β² needs to be PCR amplified. The reverse primer will have the following sequence: 1. 5β²GGCATCTA3β² 2. 5β²TAGATGCC3β² 3. 5β²GGACTCTA3β² 4. 5β²CGCATCGA3β² Identifying the […]


