6. Two experiments were conducted with an enzyme following MichaelisMenten kinetics at substrate concentrations of 0.5 g/l and 1 g/l. If the enzymatic reaction velocity increases approximately 2-fold at the […]
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DBT BET JRF 2022 Previous Year Questions and Video solution
Understanding Short Chain Variable Fragments (ScFv) of Antibodies: Composition and Function
102. Short Chain variable fragments (ScFv) of antibody are composed of : A. ππ» and ππΏ domains with πΆπ» domain B. ππ» and ππΏ domains along with Fc region C. […]
DBT BET JRF 2022 Previous Year Questions and Video solution
How to Interpret SDS-PAGE vs Native-PAGE Results: Understanding Protein Structure
101. A protein sample isolated from natural source was electrophoresed on a non-reducing SDSPAGE. It migrated as a band corresponding to 60 kDa. When the same protein was electrophoresed on […]
DBT BET JRF 2018 Previous Year Questions and Video solution
How to Calculate Solvent-to-Broth Ratio in Single-Step Extraction Using Partition Coefficient
5. Solvent extraction using ethanol is used to purify a target metabolite from an aqueous broth. The partitioning coefficient is 3. The ratio of solvent to broth that is required […]
DBT BET JRF 2022 Previous Year Questions and Video solution
Mycoplasma: The Exception Among Intracellular Parasites
100. Which one of the following organisms is NOT an obligate intracellular parasite and, therefore, can grow on bacteriological media? A. Mycoplasma B. Chlamydia C. Rickettsia D. Adenovirus Detailed Explanation: […]
DBT BET JRF 2018 Previous Year Questions and Video solution
How to Calculate Fold Purification and Percentage Recovery in Enzyme Purification
4. The protein concentration and enzyme activity in 100 mL of a cell free extract is 5 mg/mL and 2 units/mL, respectively. After multiple steps of purification, the final 10 […]
DBT BET JRF 2018 Previous Year Questions and Video solution
How to Calculate the Minimum Dose of a Natural Preservative with Degradation Over Time
3. An essential oil has antimicrobial activity with an MIC of 15 mg/Kg and can be used as a bio preservative. This activity is lost at the rate of 1% […]
DBT BET JRF 2022 Previous Year Questions and Video solution
X-linked Agammaglobulinemia: Understanding the Role of BTK Gene Mutation in Immunodeficiency
99. Which one of the immunodeficiency is caused by a mutation in BTK gene: A. X-linked SCID B. X-linked agammaglobulinemia C. DiGeorge syndrome D. Chronic granulomatous disease Detailed Explanation: Immunodeficiencies […]
DBT BET JRF 2018 Previous Year Questions and Video solution
How to Calculate Moisture Loss Through Packaging Using Water Vapour Transmission Rate (WVTR)
2. Water vapour transmission rate of a packaging film is 4g/m2 /day. A food product is packed in a rectangular pouch measuring 0.12m x 0.16m. The maximum amount of moisture […]
DBT BET JRF 2022 Previous Year Questions and Video solution
Understanding Peptide Bond Characteristics: Bond Lengths and Double Bond Character
98. The CO-NH moiety between two C-alpha atoms constitutes the Peptide bond. The measured bond lengths (Angstroms) are: C(alpha)-CO (1.51); C=O (1.24); C-N(1.33); N-C(alpha)1.46. In which of these, there is […]
