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20. In order to ensure that only fully processed mature mRNAs are allowed to be exported to cytosol, pre- mRNAs associated with snRNPs are retained in the nucleus. To demonstrate […]

19. Which one of the following mRNAs is most likely to be exported out of the nucleus? (1) Spliced RNA associated with the poly A binding and cap binding complex. […]

18. Pre-mRNAs are rapidly bound by snRNPs which carry out dual steps of splicing that removes the intron and joins the upstream and downstream exons. The following statements describe some […]

17. Which of following is reactive centre for splicing of exons during processing of m-RNA? (1) U1 and U5 (2) Branch point, U2 and U6 (3) Branch point, U4 and […]

16. A 1257 bp genomic DNA sequence of a prokaryotic gene was cloned under a strong constitutive promoter along with a suitable polyA signal and used for development of transgenic […]

15. Regarding gene expression of eukaryotes, which statement is correct? (1) m-RNA and template DNA are has same sequence (2) m-RNA and protein synthesis can occur simultaneously (3) processing of […]

The Role of Cyclin T1 in the Human P-TEFb Complex: Key Regulator of Transcription Elongation 14. The positive transcription elongation factor. P-TEFb, is a Mufti-protein complex that plays an essential […]

13. Which one of the following statements about eukaryotic RNA processing is INCORRECT? (1) Termination of transcription occurs co- transcriptionally at the polyadenylation site. (2) Phosphorylation of the Ser 2 […]

12. The 3’ end of most eukaryotic mRNAs is defined by the addition of a polyA tail- a processing reaction called polyadenylation. The addition of poly A tail is carried […]

11. You are working with an in vitro eukaryotic transcription system, which produced both capped and uncapped mRNAs. You incubated these mRNAs with mammalian cell nuclear extract and then quantified […]