During elongation step or protein synthesis, translocation moves the mRNA and the peptidyl t-RNA by one codon through the ribosome. Translocation in E.coli involves GTP and EF-G. However, in vitro […]
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CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Protein Synthesis and Trypsin Digestion: Which Peptide Fragment Carries Maximum 14C Label at Early Synthesis Stage?
A protein has 4 equally spaced trypsin sensitive sites which results in peptide fragments A1, A2, A3, A4 and A5 upon digestion with trypsin. Peptides A2 and As, represents N-terminal […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
The Science of Color Development in E. coli: LacZ, X-gal, ONPG, and MacConkey Agar Explained
19. Which one of the following statements relating to the mechanism of color development in response to LacZ expression in Escherichia coli is INCORRECT? (1) E. coli growth on LB […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Decoding lac Operon Merodiploid Expression: Which Combinations of I, O, Z, and Y Mutations Are Correct?
The following statements are made with respect to merodiploids of the lac operon, where “I” is the lac repressor, “O” is the lac operator, “Z” is the lacZ gene encoding […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Ribosome Recycling in Bacteria: The Essential Roles of RRF and EF-G
For continuation of protein synthesis in bacteria, ribosome need to be released from the mRNA as well as to dissociate into subunits. These processes do not occur spontaneously. They need […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Phenotype of E. coli I– O+ Z+ Y– / F’ I+ OC Z– Y+ Merodiploid: Constitutive or Inducible?
20. With reference to lac operon, what will be the phenotype of an E. coli strain having a genotype I– O+ Z+ Y– /F’ I+ OC Z– Y+ ? (1) […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Translation Initiation Factor IF3: The Anti-Association Factor for 30S and 50S Ribosomal
Which one of the following translation factors is used at the step Of translation initiation and defined as anti- association factor for 30S and 50S subunit interactions? (1) IF1 (2) […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
LacZ Expression in E. coli Detection by X-gal, ONPG, and MacConkey Agar
19. Which one of the following statements relating to the mechanism of color development in response to LacZ expression in Escherichia coli is INCORRECT? (1) E. coli growth on LB […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Understanding lacY Mutants in E. coli: β-Galactosidase Synthesis and Lactose Permease Function
Mutants of lac Y (Y–) gene of E. coli do not synthesize the lactose permease protein. The following statements refer to the behaviour of lac mutants under different experimental conditions. […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
How Internal Ribosomal Entry Sites Promote Translation Under Stress
Presence of an internal ribosomal entry site (IRES) in mRNA (1) Inhibit its translation (2) promotes its post transcriptional processing (3) has no impact on its translation (4) promotes its […]
