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Q.43. A PCR in a 𝟏𝟎𝟎𝝁 𝐋 reaction volume, containing two primers at a concentration of 𝟎. 𝟐𝝁𝐌 each, is set up to amplify a 250 base pair DNA fragment. […]

Q.42. The second pKa of phosphoric acid is 6.8. The ratio of 𝐍𝐚𝟐𝐇𝐏𝐎𝟒 to 𝐍𝐚𝐇𝟐𝐏𝐎𝟒 required to obtain a buffer of pH 7.0 is ____ (rounded off to two decimal […]

Q.41. A solution absorbs 𝟐𝟎% of the incident light in a cuvette of path length 1.0 cm . The amount of light transmitted by the same solution in a cuvette […]

Q.40. Polypeptides are either biosynthesized on the ribosomes using an mRNA template or chemically synthesized by the Merrifield’s solid phase method. The correct directions of peptide synthesis are (A) C […]

Q.39. The correct combination of glycosidic linkages present in glycogen is (A) 𝛼, 1 → 4 and 𝛼, 1 → 6 (B) 𝛼1 → 4 and 𝛽1 → 6 (C) […]

Q.38. Match the items in Group I with the most appropriate items in Group II and choose the correct option. Group I                  […]

Q.37. The prosthetic groups/cofactors involved in both 𝟏𝐞−and 𝟐𝐞−transfer in the mitochondrial electron transport chain are (A) NAD and NADP (B) NAD and FAD (C) Heme and FMN (D) Coenzyme […]

Q.36. One mole of a native protein upon N-terminal analysis yielded one mole each of Asp and Val. Therefore, the protein in its native state exists as a (A) Monomer […]

Q.NO.35. Considering that the three pKas of histidine are pK1=1.8, pK2=9.2 and pKR=6.O, its isoelectric will be ____________ (rounded off to one decimal place). The isoelectric point (pI) of histidine, […]

Q34. When one glucose unit from glycogen gets converted to lactate in the muscle, the net number of ATP molecules produced is ______. Pathway Breakdown Glycogen breakdown in muscle begins […]