Q.18 Which type of polyacrylamide gel can be used for analyzing the four different proteins listed below? Protein P: 60kDa, pI4 Protein Q: 45 kDa , pI 8 Protein R: […]
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Protein Sequencing Techniques
Q.17 Which one of the following techniques CANNOT be used to determine the sequence of a novel protein? (A) De novo sequencing by ESI-MS/MS (B) Edman degradation (C) Sanger sequencing […]
Protein Molecular Weight Native
Q.16 The molecular weight of a protein as determined by native PAGE is 400 kDa . This protein when run on a non-reducing SDS-PAGE gave a band of 200 kDa […]
Pinacol Rearrangement Explained
Q.15 The major product in the following reaction is Major Product: Option (B) The reaction involves acid-catalyzed dehydration of 2-methylcyclohexane-1,2-diol using p-TsOH in benzene with heat, proceeding via pinacol rearrangement […]
Monochlorination Isomers of 1-Bromopropane
Q.14 If mono-chlorination occurs at every carbon in the following reaction, the number of isomers (stereo isomers + constitutional isomers) that one can have is (A) 4 (B) 5 (C) […]
Nucleophilic Substitution in Alkyl Bromides
Q.13 The type of nucleophilic substitution and the possible products for each of the reactions 𝑷 and 𝑸 are: Nucleophilic Substitution Mechanisms in Reactions P and Q Reaction P involves […]
Protein Molecular Weight from Osmotic Pressure
Q.12 One gram of a protein is dissolved in one liter of water. The resulting solution exerts an osmotic pressure of 1.4 Torr at 298 K. Assuming that the protein […]
EMF of Ag(s)|Ag+(0.01 M)||Ag+(0.1 M)|Ag(s) Cell
Q.11 The EMF of the following cell at 298.15 K is Ag(s)|Ag+(0.01 M)||Ag+(0.1 M)|Ag(s) (Standard reduction potential for Ag+ + e– → Ag = -0.80 V) (A) 0.12 V (B) […]
Determining Overall Order of Reaction
Q.10 For a reaction A + B → products, the following data was obtained: [A] (M) [B] (M) Initial rate 0.1 0.1 r 0.2 0.1 4r [A]₀ and [B]₀ are […]
First Ionization Energy of Helium Atom
Q.9 Assuming independent non-interacting electrons, the first ionization energy of Helium atom is (A) 13.6 eV (B) 27.2 eV (C) 54.4 eV (D) 108.8 eV Answer: 54.4 eV (Option C) […]


