127. A diafiltration process is used to remove salts from a protein solution of 1L. After 1L of makeup buffer (without salts) has been added to this system, the residual […]
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DBT BET JRF 2018 Previous Year Questions and Video solution
Title: The Role of Aminopterin in HAT Medium and Its Specific Target in Cell Fusion Selection
59. Aminopterin in HAT medium inhibits: 1. Thymidine kinase 2. Hypoxanthine – guanine phosphoribosyltransferase 3. Ribonucleotidereductase 4. Dihydrofolatereductase Aminopterin in HAT Medium: Inhibiting DNA Synthesis for Hybridoma Selection In the […]
DBT BET JRF 2018 Previous Year Questions and Video solution
Why a Cloned Human Gene in E. coli May Show Poor Protein Expression Despite High mRNA Levels
58. A human gene is cloned in an E. coli expression vector. However, extremely poor protein expression is detected on SDS-PAGE despite high levels of specific mRNA. The most likely […]
DBT BET JRF 2018 Previous Year Questions and Video solution
Calculating Insert and Vector Quantities in Ligation Reactions
57. A 1 Kb insert (I) is ligated to a 5 Kb vector (V) in a molar ratio of I:V of 5:1, in a reaction volume of 1 mL at […]
DBT BET JRF 2022 Previous Year Questions and Video solution
Understanding Giardiasis: Site of Infection and Clinical Manifestation
126. Which one of the following is the most accurate statement regarding giardiasis? A. The drug of choice for giardiasis is chloroquine. B. Giardia lamblia produces an enterotoxin that increases […]
DBT BET JRF 2018 Previous Year Questions and Video solution
What Is Hot Start PCR and Why Is It Used?
56. Hot start PCR is performed to 1. Expedite the PCR reaction 2. Prevent mutations 3. Prevent primer – dimer formations 4. Minimize non-specific amplification Hot Start PCR: Purpose and […]
DBT BET JRF 2022 Previous Year Questions and Video solution
Understanding MacConkey Agar: Selectivity and Bacterial Growth
125. Given below are two statements: Statement I: MacConkey agar is a media used for recovery of Gram positive bacteria from mixed bacterial culture. Statement II: The bile salts and […]
DBT BET JRF 2018 Previous Year Questions and Video solution
Techniques for Determining Hemoglobin Subunit Stoichiometry
55. Hemoglobin is made up of two copies each of α and β globin subunits. The techniques used to establish the stoichiometry of α and β subunits are: 1. SDS […]
DBT BET JRF 2018 Previous Year Questions and Video solution
The Role of the TAP Complex in MHC Class I Antigen Presentation
54. The transporter associated with antigen processing (TAP) complex is necessary for the loading of peptides onto class I MHC molecules. The cellular compartment harboring the TAP complex is: 1. […]
DBT BET JRF 2022 Previous Year Questions and Video solution
What Gives Beetroot Its Red Color? Exploring the Pigments
124. The red color of beetroot is due to the presence of: A. Betalains B. Anthocyanins C. Flavonoids D. Carotenoids Detailed Explanation: The red color of beetroot comes from a […]
