67. A Yeast plasmid vector has an XhoI site in the LEU2 marker gene and a SalI site in the HIS3 marker gene. A student cloned a gene using the […]
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DBT BET JRF 2018 Previous Year Questions and Video solution
Troubleshooting Non-Recombinant Clones in Cloning with HindIII and EcoRI
65. A DNA fragment digested with HindIII and EcoRI was ligated with a vector digested with HindIII and EcoRI sites present in the polylinker of the vector. Upon screening of […]
DBT BET JRF 2018 Previous Year Questions and Video solution
Effect of Medium Shift on Bacillus Growth: Genome, Transcriptome, and Proteome Changes
64. A Bacillus culture growing in a rich medium was shifted to a sporulation medium. Which of the following is expected to occur? 1. Genome and transcriptome will change 2. […]
DBT BET JRF 2019 Previous Year Questions and Video solution
Mechanism of mRNA Transcription Termination in Saccharomyces cerevisiae
63. Termination of mRNA transcription in Saccharomyces cerevisiae is mediated by: 1. Rho-dependent termination 2. Rho-independent termination 3. Polyadenylation signal 4. Sigma factor directed termination Introduction In Saccharomyces cerevisiae, a […]
DBT BET JRF 2022 Previous Year Questions and Video solution
Specific Growth Rate Pattern in Fed-Batch Reactors with Concentrated Substrate Feed
130. A concentrated feed of substrate is added at a constant rate in a fed- batch reactor. If all the substrate is utilized, the specific growth rate (𝜇) pattern will […]
DBT BET JRF 2018 Previous Year Questions and Video solution
: Understanding Merodiploids in the Lac Operon: Which is NOT Inducible by Lactose?
62. Among the merodiploids of the lac operon in E. coli, which one is NOT inducible by lactose? 1. i – p o z/ i p o z 2. i […]
DBT BET JRF 2018 Previous Year Questions and Video solution
Understanding Repressor Binding Dynamics: Calculating Occupancy Based on KD and Concentration
61. In a cell, a repressor binds to its cognate operator with a KD = 10-9M. The cellular concentration of the repressor is 10-8M. The extent of repressor bound to […]
DBT BET JRF 2022 Previous Year Questions and Video solution
Understanding Histone-DNA Binding: The Role of Amino Acid Composition
129. Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R. Assertion A: Histones bind to DNA with high affinity. Reason […]
DBT BET JRF 2018 Previous Year Questions and Video solution
: Optimal DNA Concentration for Maximum Self-Circularization Yield Using T4 DNA Ligase
60. A linear double stranded DNA is self-circularized by ligation with T4 DNA ligase. At which concentration (μg/ml) of DNA would the self-circularization yield be maximum? 1. 30 2. 10 […]
DBT BET JRF 2022 Previous Year Questions and Video solution
Best One-Step Purification Technique for Histone-Binding Proteins from Native Sources
128. A laboratory is trying to purify a Histone-binding protein from its native source. Which one of the following purification techniques should be used for one-step purification of the protein? […]
