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Q.63 Consider a simple uni-substrate enzyme that follows Michaelis-Menten kinetics. When the enzyme catalyzed reaction was carried out in the presence of 10 nM concentration of an inhibitor, there was […]

Q.62 First order deactivation rate constants for soluble and immobilized amyloglucosidase enzyme are 0.03 min-1 and 0.005 min-1, respectively. The ratio of half-life of the immobilized enzyme to that of […]

Q.61 Consider the following alignment of two DNA sequences: AGTAAC AA—-AC Assuming an affine gap scoring scheme of an identity matrix for substitution, a gap initiation penalty of 1 and […]

Q.60 At the end of a batch culture, glucose solution is added at a flow rate of 200 ml/h. If the culture volume after 2 h of glucose addition is […]

Q.59 Yeast converts glucose to ethanol and carbon dioxide by glycolysis as per the following reaction: C6H12O6 → 2C2H5OH + 2CO2 Assuming complete conversion, the amount of ethanol produced (in […]

Q.58 Mammalian cells in active growth phase were seeded at a density of 1×105 cells/ml. After 72 hours, 1×106 cells/ml were obtained. The population doubling time of the cells in […]

Q.57 In a chemostat, the feed flow rate and culture volume are 100 mL/h and 1.0 L, respectively. With glucose as substrate, the values of μmax and Ks are 0.2 […]

Q.56 A microbial strain is cultured in a 100 L stirred fermenter for secondary metabolite production. If the specific rate of oxygen uptake is 0.4 h-1 and the oxygen solubility […]

Q.55 An aqueous solution containing 6.8 mg/L of an antibiotic is extracted with amyl acetate. If the partition coefficient of the antibiotic is 170 and the ratio of water to […]

Q.54 Moist heat sterilization of spores at 121 °C follows first order kinetics as per the expression: dN/dt = -kdN where, N is the number of viable spores, t is […]