21. Co-localization of two fluorescently labelled proteins in an organelle in cells is usually visualized by: A. Interference-contrast microscopy B. Scanning electron microscopy C. Confocal microscopy D. Atomic force microscopy […]
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Microscopic Techniques Matching
20. Match the following microscopic techniques with their respective functions Microscopic technique Functions 1. Phase contrast microscopy 2. Fluorescent microscopy 3. Transmission electron microscopy 4. Bright field microscopy 5. Dark […]
SEM Image Formation Electrons Scattered Surface
19. In scanning electron microscope (SEM) to form an image of the specimen, (A) An electron should pass through the specimen (B) Electrons are scattered from the surface of the […]
FRAP Nuclear Protein Movement
18. The movement of proteins within the nucleus can be determined by: A. Electron microscopy B. Fluorescence recovery after photobleaching C. Fluorescent in situ hybridization D. Confocal light microscopy Fluorescence […]
Confocal Microscopy Techniques
17. You wish to localize a given gene product at subcellular levels following immunofluorescence staining. Routine microscopy could not resolve whether the gene product is localized inside the nucleus or […]
Fluorescence Microscopy Cell Tracking Reporter Gene
16. The movement of a single cell was required to be continually monitored during development. This cell was marked with a reporter gene. To visualize this movement one would use: […]
Bindin Protein Expression Monitoring
15. Bindin is a protein that is expressed in the tip of the sperm head (acrosome) and is important for sperm–egg interaction. Its distribution pattern changes during sperm maturation. Which […]
Microscopy Technique for Transmembrane Proteins
14. Which one among the following microscopic techniques is used to see clear resolution for the topology and distribution of transmembrane proteins of a cell membrane? A. Freeze-fracture electron microscopy […]
Scanning Tunneling Microscopy Weakness
13. What is considered a weakness of scanning tunneling microscopy (STM)? A. The inability to move and arrange atoms to create a design B. The requirement for a conducting surface […]
Fluorescence Microscopy Principle
12. Fluorescence microscopy is based on the ability of certain molecules to: A. Absorb light of a constant wavelength B. Absorb light of many different wavelengths C. Absorb light at […]


