Electrophoresis of a purified protein in SDS-PAGE in the presence of b-marcaptoethanol yields two bands of 35 kDa and 45 kDa. However, in a gel filtration chromatography, the same protein […]
Blog
Education
Separated the two proteins from the mixture one is monomeric
What method we should use to separated the two proteins from the mixture in which one is monomeric with MW of 10 kDa and other is of two Homodimer’s with […]
CSIR NET Previous Year Questions Gel Electrophoresis
An oligonucleotide bound to a protein migrates slower than the free oligonucleotide in a polyacrylamide gel
An oligonucleotide bound to a protein migrates slower than the free oligonucleotide in a polyacrylamide gel. If antibody specific to the oligonucleotide binding protein is bound to this complex, the […]
CSIR NET Previous Year Questions Gel Electrophoresis
Chymotrypsinogen in the native state and reduced unfolded sates is loaded- Gel-electrophoresis
Chymotrypsinogen in the native state and reduced unfolded sates is loaded in well A and B respectively. They are electrophoreses and stain, one of the following results is likely to […]
CSIR NET Previous Year Questions Gel Electrophoresis
Proteins A and B having a molecular weight of 16500 and 35400 move 1.3 cm and 4.6 cm
Proteins A and B having a molecular weight of 16500 and 35400 move 1.3 cm and 4.6 cm, respectively, when electrophoresed through a gel. What is the molecular weight of […]
CSIR NET Previous Year Questions Gel Electrophoresis
The relative electrophoretic mobilities of a 30-kd protein and a 92-kd protein used as standards on an SDS
The relative electrophoretic mobilities of a 30-kd protein and a 92-kd protein used as standards on an SDS polyacrylamide gel are 0.80 and 0.41, respectively. What is the apparent mass […]
CSIR NET Previous Year Questions Gel Electrophoresis
What is an issue with using 2D-PAGE
What is an issue with using 2D-PAGE? a. Hydrophobic proteins may not run as expected due to the hydrophobic surfaces. b. Some proteins may not migrate through polyacrylamide and therefore […]
CSIR NET Previous Year Questions Gel Electrophoresis
SDS Used in the Electrophoresis of Proteins
Why is SDS used in the electrophoresis of proteins? a. SDS coats the protein with a negative charge so that the sample can run through the gel. b. SDS is […]
CSIR NET Previous Year Questions Gel Electrophoresis
Important for Gel Electrophoresis to work
Which of the following is important for gel electrophoresis to work? a. Negatively charged nucleic acids to migrate through the gel. b. Ethidium bromide to provide a means to visualize […]
CSIR NET Previous Year Questions Gel Electrophoresis
Prepare RNA for Northern blot analysis
A researcher needs to prepare RNA for Northern blot analysis. Initial experiments using total RNA produce no signal when the experiment is completed. A method to increase the sensitivity of […]
