The Michaelis-Menten equation can be represented as follows: v = Vmax. [S]/1 + [S]/Km For an intracellular enzyme to function optimally, which of the following statements regarding intracellular substrate concentration […]
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Amylopectin Branch Points
If a branched polysaccharide (e.g. amylopectin) has 7 branch points, how many free -OH ends are available for reduction? 7 1 6 0 The correct answer is 1. In amylopectin, […]
Tyrosine to Histidine Mutation in Enzyme Active Site
A tyrosine-to-histidine mutation in the active site of an enzyme significantly affects its activity. The reaction is likely to be Acid-catalysed Base-catalysed Free radical-catalysed None of the above A tyrosine-to-histidine […]
Microwave Rotational Spectrum
Select one of the below sets in which all the molecules show a microwave rotational spectrum? H2, CH4, SF6 HCl, CH3Cl, CH2Cl2, H2O H2, HCl, CH4, CH3Cl CH2Cl2, H2O, SF6 […]
Polypeptide 11 kDa to ORF Mass
If you have a polypeptide of size 11 kilo Daltons, what would be the mass of the open reading frame that codes for the polypeptide? The average mass of an […]
Phosphoric Acid Predominant Form at pH 3.2
Phosphoric acid is tribasic with three pKa values of 2.14, 6.86, and 12.4, respectively. The ionic form of the acid that predominates at pH 3.2 is: PO43- HPO42- H2PO4- H3PO4- […]
Enzyme Velocity vs Substrate Concentration
When the velocity of enzyme activity is plotted against substrate concentration, which of the following is obtained? Hyperbolic curve Parabola Straight line with positive slope Straight line with negative slope […]
Protein Molar Ratio Calculation
20 μl of a 10 mg/ml solution of protein A (molecular weight 10 kDa) is added to 20 μl of a 10 mg/ml solution of protein B of molecular weight […]
Protein Conformation at Physiological Temperature
The melting temperature of a human protein is 33 °C in 100 mM sodium phosphate buffer at pH 6.5. What should the predominant conformation of the protein in the same […]
Maximizing NO2 Yield from N2O4
Dinitrogen tetroxide (N2O4) breaks down into nitrogen dioxide (NO2). If the reaction is reversible and endothermic, which condition will give the largest yield of NO2? High temperature and high pressure. […]


