Q.33 Match the promoters listed in Group I with the tissues listed in Group II
| Group I | Group II |
|---|---|
| P. α-Amylase | 1. Endosperm |
| Q. Glutenin | 2. Tuber |
| R. Phaseollin | 3. Aleurone |
| S. Patatin | 4. Cotyledon |
Options:
(A) P-3, Q-1, R-4, S-2
(B) P-3, Q-4, R-1, S-2
(C) P-4, Q-2, R-1, S-3
(D) P-1, Q-3, R-2, S-4
Introduction
Tissue-specific promoters are widely used in plant biotechnology to control gene expression in particular organs or tissues. This question tests knowledge of seed-specific, tuber-specific, and defense-related promoters, which is essential for understanding transgenic crop development.
Step-by-Step Matching Explanation
P. α-Amylase → 3. Aleurone
α-Amylase is expressed in the aleurone layer of cereal seeds, where it helps break down starch during seed germination.
✅ Correct match: P-3
Q. Glutenin → 1. Endosperm
Glutenin is a major storage protein of wheat, expressed specifically in the endosperm.
✅ Correct match: Q-1
R. Phaseollin → 4. Cotyledon
Phaseollin is a storage and defense protein found mainly in the cotyledons of legumes (e.g., bean seeds).
✅ Correct match: R-4
S. Patatin → 2. Tuber
Patatin is a major storage protein of potato tubers, making it a classic tuber-specific promoter.
✅ Correct match: S-2
Correct Matching Sequence
P-3, Q-1, R-4, S-2
Correct Answer
✅ Option (A)
Explanation of All Options
Option (A): P-3, Q-1, R-4, S-2
✔ Correct
All promoters are accurately matched with their known tissue-specific expression.
Option (B): P-3, Q-4, R-1, S-2
❌ Incorrect
-
Glutenin is not expressed in cotyledons
-
Phaseollin is not an endosperm protein
Option (C): P-4, Q-2, R-1, S-3
❌ Incorrect
-
α-Amylase is not cotyledon-specific
-
Patatin is not expressed in aleurone
Option (D): P-1, Q-3, R-2, S-4
❌ Incorrect
-
α-Amylase is not an endosperm promoter
-
Glutenin is not aleurone-specific
-
Patatin is not cotyledon-specific
Quick Memory Tricks
-
α-Amylase → Aleurone (starch digestion during germination)
-
Glutenin → Endosperm (wheat storage protein)
-
Phaseollin → Cotyledon (legume storage/defense protein)
-
Patatin → Tuber (potato storage protein)
Conclusion
The correct matching of promoters with tissues is P-3, Q-1, R-4, S-2, which corresponds to Option (A). Understanding tissue-specific promoters is crucial for designing effective plant genetic engineering strategies.