Q.33 Match the promoters listed in Group I with the tissues listed in Group II Group I Group II P. α-Amylase 1. Endosperm Q. Glutenin 2. Tuber R. Phaseollin 3. Aleurone S. Patatin 4. Cotyledon Options: (A) P-3, Q-1, R-4, S-2 (B) P-3, Q-4, R-1, S-2 (C) P-4, Q-2, R-1, S-3 (D) P-1, Q-3, R-2, S-4

Q.33 Match the promoters listed in Group I with the tissues listed in Group II

Group I Group II
P. α-Amylase 1. Endosperm
Q. Glutenin 2. Tuber
R. Phaseollin 3. Aleurone
S. Patatin 4. Cotyledon

Options:

(A) P-3, Q-1, R-4, S-2

(B) P-3, Q-4, R-1, S-2

(C) P-4, Q-2, R-1, S-3

(D) P-1, Q-3, R-2, S-4


Introduction

Tissue-specific promoters are widely used in plant biotechnology to control gene expression in particular organs or tissues. This question tests knowledge of seed-specific, tuber-specific, and defense-related promoters, which is essential for understanding transgenic crop development.


Step-by-Step Matching Explanation

P. α-Amylase → 3. Aleurone

α-Amylase is expressed in the aleurone layer of cereal seeds, where it helps break down starch during seed germination.

Correct match: P-3


Q. Glutenin → 1. Endosperm

Glutenin is a major storage protein of wheat, expressed specifically in the endosperm.

Correct match: Q-1


R. Phaseollin → 4. Cotyledon

Phaseollin is a storage and defense protein found mainly in the cotyledons of legumes (e.g., bean seeds).

Correct match: R-4


S. Patatin → 2. Tuber

Patatin is a major storage protein of potato tubers, making it a classic tuber-specific promoter.

Correct match: S-2


Correct Matching Sequence

P-3, Q-1, R-4, S-2


Correct Answer

Option (A)


Explanation of All Options

Option (A): P-3, Q-1, R-4, S-2

Correct
All promoters are accurately matched with their known tissue-specific expression.


Option (B): P-3, Q-4, R-1, S-2

❌ Incorrect

  • Glutenin is not expressed in cotyledons

  • Phaseollin is not an endosperm protein


Option (C): P-4, Q-2, R-1, S-3

❌ Incorrect

  • α-Amylase is not cotyledon-specific

  • Patatin is not expressed in aleurone


Option (D): P-1, Q-3, R-2, S-4

❌ Incorrect

  • α-Amylase is not an endosperm promoter

  • Glutenin is not aleurone-specific

  • Patatin is not cotyledon-specific


Quick Memory Tricks

  • α-Amylase → Aleurone (starch digestion during germination)

  • Glutenin → Endosperm (wheat storage protein)

  • Phaseollin → Cotyledon (legume storage/defense protein)

  • Patatin → Tuber (potato storage protein)


Conclusion

The correct matching of promoters with tissues is P-3, Q-1, R-4, S-2, which corresponds to Option (A). Understanding tissue-specific promoters is crucial for designing effective plant genetic engineering strategies.

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