52. Enzyme-linked immunospot (ELISPOT) assay measures: (a)Cytokine concentration in culture supernatant (b)Expression of cytokine gene (c)Intracellular cytokine concentration (d)Number of cytokine releasing cells at single cell level Article: Introduction The […]
Tag: dbt jrf pyq
Tag: dbt jrf pyq
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DNA denaturation cooperative phenomenon
Denaturation of DNA is a 1. temperature-independent process 2. linear process 3. cooperative phenomenon 4. neither linear nor a cooperative process What is DNA Denaturation? DNA denaturation refers to the […]
Why You Don’t Need Topoisomerase in a PCR Reaction Mix for Gene Amplification
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- April 13, 2025
- 4 Comments
51. DNA replication requires DNA-Topoisomerase to remove the supercoiling of DNA that accumulates at the end of a growing replication fork. You wish to perform a PCR amplification of a […]
Which Aminoacyl-tRNA Binds First to the P Site During Bacterial Translation?
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- April 13, 2025
- 3 Comments
50. During translation which one of the following aminoacyl-tRNA binds first to the P site of bacterial ribosomes? (a) fMet-tRNAfMet (b) fMet-tRNAMet (c) Met-tRNAfMet (d) Met-tRNAMet Article: Introduction In the […]
Understanding Why Sucrose is Non-Reducing Sugar
Which of the following is a non-reducing sugar? 1. fructose 2. galactose 3. ribose 4. sucrose What is a Non-Reducing Sugar and Why is Sucrose One? In the world of […]
What Is the Shortest Land Route Between Mumbai and Mexico on the Same Latitude?
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- April 13, 2025
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49. The shortest land route has to be determined from Mumbai to a city X in Mexico at the same latitude. The route will be: (a)A line curving so that […]
How to Separate Single-Stranded DNA from Double-Stranded DNA Using Hydroxyapatite Chromatography
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- April 13, 2025
- 11 Comments
Single stranded DNA can be separated from double stranded DNA efficiently using 1. hydrophobic interaction chromatography 2. RP-HPLC 3. hydroxyapatite chromatography 4. urea PAGE How to Separate Single-Stranded DNA […]
Understanding Seating Arrangements: Solving Complex Constraints in Seating Puzzles
A, B, C, and D are to be seated in a row, But C and D cannot be together. Also B cannot be at the third place. Which of the […]
Why Trypsin Cleavages at Arginine and Lysine Residue amino acid
Trypsin cleaves a protein at the 1. C – terminus side of Arg/Lys residues 2. N – terminus side of Arg/Lys residues 3. C – terminus side of Val/lle residues […]
Understanding the Differences Between Prokaryotic and Eukaryotic Cells
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- April 13, 2025
- 2 Comments
Which of the following is NOT true ? 1. prokaryotes are unicellular organisms 2. eukaryotes can be either multicellular or unicellular organisms 3. prokaryotic cells lack nucleus whereas eukaryotic cells […]


