27. The enzyme used to prevent unwanted self-ligation of DNA molecules during cloning experiments is: (a) Alkaline phosphatase, (b) Reverse transcriptase, (c) Terminal peroxidase, (d) Terminal phosphatase Introduction In DNA […]
Tag: dbt jrf pyq
Tag: dbt jrf pyq
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The Role of Cardiolipin in Mitochondrial Membranes
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- April 13, 2025
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26. The presence of Cardiolipin is a characteristic of the membrane of: (a) Endoplasmic reticulum, (b) Lysosomes, (c) Mitochondria, (d) Myelin sheets Introduction Cardiolipin is a unique phospholipid that plays […]
Understanding the Role of FEN-1 in Degrading RNA Primers During Eukaryotic DNA Replication
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- April 13, 2025
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25. During eukaryotic replication, ________ degrades the RNA primer by 5’ – 3’ exonuclease activity. Options:- (a) DNA polymerase V, (b) FEN-1, (c) RNAseH1, (d) Topoisomerase IIB Introduction DNA replication […]
What Is Endoreduplication? Understanding Recurrent DNA Replication Without Cell Division
What is Endoreduplication? 1. replication of DNA in the nuclei and endocytosis of one copy to another organelle 2. mobilization of DNA into ER and replication of DNA in the […]
Understanding Nickel-NTA Affinity Chromatography and His-Tag Protein Purification
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- April 13, 2025
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24. A scientist chose Nickel – NTA affinity chromatography to purify a recombinant protein. Which one of the following tag was present in his recombinant protein? (a) Flag, (b) Glutathione-S-transferase, […]
Understanding Forward and Reverse Genetics in Immune Function Studies
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- April 13, 2025
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23. A geneticist interested in immune function induces random mutations in a number ofspecific genes in mice and then determines which of the resulting mutant mice have impaired immune function. […]
Understanding Gene Relationships: Paralogs and Gene Duplication Events
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- April 13, 2025
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22. Genes that are related through gene duplication events are: (a) Analogs, (b) Homologs, (c) Orthologs, (d) Paralogs Introduction Gene duplication is a fundamental mechanism in molecular evolution. It plays […]
Tay-Sachs Disease: A Genetic Disorder That Does Not Exhibit Sex-Linked Inheritance
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- April 13, 2025
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Which of the following disorders does not show sex-linked inheritance? 1. Haemophilia B 2. Duchenne muscular dystrophy 3. Haemophilia A 4. Tay-Sachs disease What Is Tay-Sachs Disease? Tay-Sachs disease is […]
Validating Protein Secondary Structure: The Role of Ramachandran Plot
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- April 13, 2025
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21. Which one of the following is used to validate the secondary structure of proteins? (a) Dot plot, (b) Neural network, (c) Ramachandran plot, (d) Sigma plot Introduction Proteins are […]
Understanding the Protein Backbone Angle: A Key to Protein Structure
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- April 13, 2025
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20. Which one of the following defines the angle in the protein backbone? (a) 𝑁 − 𝐶𝑎 − 𝐶 − 𝑁 , (b) 𝐶𝑎 − 𝐶 − 𝑁 − 𝐶 […]


