Tag: dbt jrf 2018

58. A human gene is cloned in an E. coli expression vector. However, extremely poor protein expression is detected on SDS-PAGE despite high levels of specific mRNA. The most likely […]

57. A 1 Kb insert (I) is ligated to a 5 Kb vector (V) in a molar ratio of I:V of 5:1, in a reaction volume of 1 mL at […]

56. Hot start PCR is performed to 1. Expedite the PCR reaction 2. Prevent mutations 3. Prevent primer – dimer formations 4. Minimize non-specific amplification Hot Start PCR: Purpose and […]

55. Hemoglobin is made up of two copies each of α and β globin subunits. The techniques used to establish the stoichiometry of α and β subunits are: 1. SDS […]

54. The transporter associated with antigen processing (TAP) complex is necessary for the loading of peptides onto class I MHC molecules. The cellular compartment harboring the TAP complex is: 1. […]

53. The mechanism that permits immunoglobulins to be synthesized either in a membrane bound or secreted form is: 1. Allelic exclusion 2 Co-dominant expression 3. Differential RNA processing 4. Class-switch […]

52. Which one of the following is NOT a part of the recombination signal sequence in VDJ recombination? 1. A conserved 7 bp consensus sequence 2. Locus control regions (LCR) […]

51. Which one of the following amino acid biosynthesis pathways is affected in folic acid deficiency? 1. Aspartate from oxaloacetate & glutamate 2. Glycine from glucose and alanine 3. Glutamate […]

50. In an eukaryotic pre-mRNA the number of positions where polyadenylate modifications occur is: 1. 5 2. 2 3. 3 4. 4 PART B Polyadenylation Modifications in Eukaryotic Pre-mRNA: An […]

48. Taq DNA polymerase differs from the Klenow fragment in having: 1. 5′-3′ polymerase activity 2. 5′-3′ exonuclease activity 3. 3′-5′ exonuclease activity 4. Endonuclease activity Taq DNA Polymerase vs. […]