4. DNA was isolated from a strain of bacterium with genotype a+b+c+d+e+ and transformed into a bacterial strain a–b–c–d–e-. The transformants were tested for the presence of the donated genes. […]
Tag: CSIR NET Life Science Previous Year Questions and Solution Genetics
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CSIR NET Life Science Previous Year Questions and Solution on Genetics
Determining Bacterial Gene Order via Cotransformation in Transformation Experiments
5. DNA from a bacterial strain with genotype at b+c+d+e+f+ was isolated and used to transform a strain of bacteria with genotype a–b–c–d–e–f–.The transformed cells were tested for the presence […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
Bacterial Gene Order via Cotransformation Mapping
DNA from a strain of bacteria with genotype a+b+c+d+e+ was isolated and used to transform a strain of bacteria that was a–b–c–d–e–. The transformed cells were tested for the presence […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
Selecting lac+ E. coli Mutants
2. A lac– culture of E. coli was mutagenised. On what media would one spread the mutagenised cells to select for lac+ cells? (1) Minimal media+ lactose. (2) Rich media […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
Why Same Number of Colonies in E. coli Tetracycline Transformation on LB Medium?
1. E. coli cells were transformed with plasmid carrying tetracycline resistance gene and later on plated on LB medium and various colonies were observed but same number of colonies were […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
Understanding Site-Specific Recombinases
21. Which of the following statements about site-specific recombinases is NOT true? (1) The Cre recombinase is believed to mediate the circularization of the Pl phage genome during infection of […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
Cre-LoxP System in Knockout Mice
20. Construction of knockout mice may be performed using the Cre-LoxP system. Eventually, the Cre recombinase of the bacteriophage P1 mediates site- specific recombination at a 34 bp sequence, lox […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
How LoxP Orientation Controls Inversion of the EFG Segment
19. Cre/loxP system is used by phage Pl to remove terminally redundant sequences that arise during packaging of the phage DNA. Cre-lox system can be used to create targeted deletions, […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
Enzymes Involved in Site-Specific Recombination
18. Site specific recombination results in precise DNA rearrangements, which is limited to specific sequences. The enzymes that are important to carry out the process are (1) Restriction endonuclease and […]
CSIR NET Life Science Previous Year Questions and Solution on Genetics
Cre‑Lox Strategy to Create Prostate‑Specific p53 Knockout Mice
17. In order to create an animal whose prostate gland is devoid of the p53 gene, engineered animals were created by cloning specially designed knock-out cassettes. Identify the most appropriate […]
