Reason for Multiple Bands in PCR Amplification Why a scholar always gets four bands of 0.5 kb, 1 kb, 1.5 kb and 2.0 kb respectively during PCR amplification of a […]
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CSIR NET Previous Year Questions on PCR
Principle of Real-Time PCR
Principle of Real-Time PCR The principle which real time PCR is based on; (a) Amplifying and simultaneously quantifying a target DNA (b) Analyzing the PCR product at a predetermined time […]
CSIR NET Previous Year Questions on PCR
Regulation of Heating and Cooling in PCR
Regulation of Heating and Cooling in PCR The alternate heating and cooling of reaction mixture in a PCR is regulated by (a) A heating element and refrigerant (b) A Peltier […]
CSIR NET Previous Year Questions on PCR
Reason for PCR Failure Due to Incorrect Annealing Temperature
Reason for PCR Failure Due to Incorrect Annealing Temperature A scholar set up a PCR to copy a specific gene promoter using primers based on genomic database and a new […]
CSIR NET Previous Year Questions on PCR
Selecting Primers for DNA Amplification
Selecting Primers for DNA Amplification Choose the pair of primers that will allow amplification of the DNA sequence both two given structures the DNA ? 5’GACCTGTGGAAGC…………..CATACGGGATTGA3′ 3’CTGGACACCTTCG……………GTATGCCCTAACT5′ 1. 5’GACCTGTGGAAGC3′ […]
CSIR NET Previous Year Questions on Blotting
Techniques for Diagnosing Influenza Virus Infections
Techniques for Diagnosing Influenza Virus Infections Diagnosis of influenza virus infections can be done using some of the following techniques: A. Western blot and Southern blot B. Northern blot and […]
CSIR NET Previous Year Questions on Blotting
Techniques for Analyzing Gene Expression Pattern
Techniques for Analyzing Gene Expression Pattern Which one of the following techniques/approaches CANNOT be used to analyse expression pattern of a gene? (1) Western blotting (2) qRT-PCR (3) Northern […]
CSIR NET Previous Year Questions on Blotting
AFLP technique for DNA polymorphism detection
Molecular Marker Types Using Both Restriction Enzyme and PCR Techniques Which one of the following molecular marker types uses combination of both restriction enzyme and PCR techniques? (1) SSR (2) […]
CSIR NET Previous Year Questions on Blotting
Detection of Specific mRNAs Within a Cell
Detection of Specific mRNAs Within a Cell The presence and distribution of specific mRNAs within a cell can be detected by (1) Northern blot analysis (2) RNase protection assay (3) […]
CSIR NET Previous Year Questions on Blotting
Techniques for Separation, Detection, and Visualization of DNA
Techniques for Separation, Detection, and Visualization of DNA Which one of the following techniques CANNOT be used for separation, detection or visualization of DNA? (1) Western Blotting (2) Polyacrylamide gel […]
