Amongst the following. which one is the most appropriate strategy to sequence and assemble highly repeated regions of a genome? (1) Shot gun sequencing (2) Illumina sequencing (3) 454 sequencing […]
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CSIR NET Previous Year Questions on DNA SEQUENCING
Pyrosequencing and Emulsion PCR
Which method is used to generate clonally amplified DNA fragments to be used as templates in Pyrosequencing? A. Ligation PCR B. Shot gun cloning C. BAC cloning D. Emulsion PCR […]
CSIR NET Previous Year Questions on Protein Sequencing
Reagent for Generating Peptides from Histones
Histones have very high percentage of basic amino acids (15-30%). For this class of proteins which of the following reagents would be a suitable choice for generating peptides in determination […]
CSIR NET Previous Year Questions on DNA SEQUENCING
Codon Bias and Its Relation to Species-Specific Genetic Code Usage
Codon bias related to which of the following? A. Some codons for an amino acids are more frequently used in all species B. Some amino acids are rarely used in […]
CSIR NET Previous Year Questions on DNA SEQUENCING
Effect of High Dideoxynucleotide Concentration in Chain Termination Primer
If the concentration of dideoxynucleotides was too high in a chain termination primer A. The dideoxynucleotides would inhibit the DNA polymerase proceed as the high B. The reactions would yield […]
CSIR NET Previous Year Questions on DNA SEQUENCING
Objective of Performing a Homology Search with a DNA Sequence
What is objective of performing a homology search with a DNA sequence A. To determine if any genes with similar sequences are present in the DNA databases B. To determine […]
CSIR NET Previous Year Questions on DNA SEQUENCING
Human Chromosome Mapping: Importance and Advances
Human chromosomes mapping A. Has been restricted to the sex chromosomes because of small family sizes B. Proceeded much more successfully as large numbers of DNA markers became available C. […]
CSIR NET Previous Year Questions on Protein Sequencing
Phenyl Isothiocyanate Reaction with Amino-Terminal Residues
Choose the correct statement(s) for? P. Phenyl isothiocyanate reacts with the amino-terminal residue Q. If a protein has a blocked amino-terminal residual (as does N-formyl methionine, for example), it cannot […]
CSIR NET Previous Year Questions on DNA SEQUENCING
How to Label Oligonucleotides by Polynucleotide Kinase and γ-32P ATP
How can use lable oligo nucleotide (GGATATCC) with any reagents P. [Y-32P] ATP Q. polynucleotide kinase R. DNA-dependent RNA polymerase S. DNA ligase A. P and Q B. R and […]
CSIR NET Previous Year Questions on DNA SEQUENCING
What is Sanger Sequencing
DNA sequencing by the Sanger method takes advantage of which property of DNA synthesis to generate a sequencing ladder? A. Addition of nucleotides requires a free OH group on the […]
