Q.55 In 2 N H₂SO₄, an organic compound shows fluorescence with quantum yield, ϕ = 0.42 and fluorescence rate constant, kf = 5.25 × 10⁷ s⁻¹. The observed fluorescence lifetime […]
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How Many Ribosomes Translate Single Bacterial mRNA
Q.54 In bacteria, a ribosome synthesizes a protein containing 300 amino acids from mRNA in 20 seconds. If the average lifetime of mRNA is 2 minutes, the number of ribosomes […]
Calculating Protein Oligomer Number from Native-PAGE Rf and Amino Acids
Q.53 The relationship between log(MW) [where MW – molecular weight in kDa] of a mixture of protein standards and their retention factors (Rf) obtained from native-PAGE is log₁₀(MW) = −2Rf […]
Enzyme Inhibition Ki Calculation
Q.52 The Kₘ and kcat of an enzyme are 4 mM and 0.1 μM h⁻¹ respectively. In the presence of 1.5 mM inhibitor, the Kₘ and kcat of the enzyme […]
Standard Free Energy Change for NADH Oxidation by Oxygen
Q.51 The standard oxidation potentials for oxidation of NADH and H₂O are +0.315 V and −0.815 V, respectively. The standard free energy for oxidation of 1 mole of NADH by […]
Photoelectric Effect
Q.50 Two sources P and Q produce electromagnetic waves with wavelengths Z and 2Z, respectively. Source P ejects a photon with a maximum kinetic energy of 4.0 eV from a […]
Radioactive Decay Half-Life Calculation
Q.49 One gram of radioactive nuclei with a half‑life of 300 days is kept in an open container. The weight of nuclei remaining after 900 days (correct to 1 decimal […]
How to Evaluate ∫01 2x dx + ∫12 (2 − x) dx
Q.48 ∫₀¹ z dx + ∫₁² (2 − x) dx = ________. The expression in the question is mathematically incomplete as shown in the image, so the exact integral cannot […]
Positive Root of x³ + x² – 2 = 0
Q.47 The positive root of the equation x³ + x² − 2 = 0 is ________. The positive root of the equation x3+x2−2=0x3+x2−2=0 is exactly 1. Solving the Equation Apply the Rational […]
Calculate pH of 0.1 N Acetic Acid with [H⁺] 1.33 × 10⁻³ M
Q.46 The [H⁺] of 0.1 N acetic acid solution is 1.33 × 10⁻³. The pH of the solution (correct to two decimal places) is ________. The pH of the 0.1 […]


