Q89. Assume that the anticodon for an unknown amino acid is 3ʹ AUG 5ʹ. The
corresponding code on DNA sequence would be
(A) 3ʹ TAC 5ʹ
(B) 5ʹ TAG 3ʹ
(C) 3ʹ ATG 5ʹ
(D) 5ʹ ATG 3ʹ
The correct answer is (A) 3ʹ TAC 5ʹ.
Anticodons on tRNA pair antiparallel with mRNA codons during translation, and the question asks for the corresponding DNA code, which refers to the template strand sequence.
Step-by-Step Reasoning
The anticodon is given as 3ʹ-AUG-5ʹ, so it pairs with the mRNA codon 5ʹ-UAC-3ʹ via complementary base pairing (A-U, U-A, G-C).
mRNA is transcribed from the DNA template strand, which must be complementary and antiparallel: for 5ʹ-UAC-3ʹ mRNA, the template DNA is 3ʹ-ATG-5ʹ? No—for mRNA 5ʹ-U A C-3ʹ, template DNA reads 3ʹ-A T G-5ʹ (T pairs with A/U, A with T, G with C). Wait, correction: RNA U from DNA A (template), so template base opposite mRNA U is A; opposite A is T; opposite C is G. Thus, template DNA is 3ʹ-ATG-5ʹ. But options lack this exactly? Standard: template DNA 3ʹ-ATG-5ʹ transcribes to mRNA 5ʹ-UAC-3ʹ.
In DNA notation, strands are often written 5ʹ→3ʹ, but here options match directions given. The “corresponding code” means the template strand triplet that dictates this codon.
Option Analysis
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(A) 3ʹ TAC 5ʹ: Incorrect for template; this is coding/sense strand 3ʹ→5ʹ (or reverse of usual 5ʹ-ATG-3ʹ coding). But wait, for codon UAC, coding DNA is 5ʹ-TAC-3ʹ (same as mRNA with T/U), template 3ʹ-ATG-5ʹ. Not matching A directly? Re-eval: actually, mistake—mRNA 5ʹ-UAC-3ʹ means template DNA base-by-base: position1 (5ʹU): template 3ʹA; pos2 A: template T; pos3 C: template G—so 3ʹ-ATG-5ʹ template. None match ATG 3ʹ-5ʹ exactly? Option C is 3ʹ ATG 5ʹ—yes!
Correction from logic and sources: anticodon 3ʹAUG5ʹ pairs mRNA codon 5ʹUAC3ʹ. DNA template that produces mRNA 5ʹUAC3ʹ is 3ʹATG5ʹ (A→U, T→A, G→C). So (C) 3ʹ ATG 5ʹ.
But PYQ lists options, and standard biology confirms template is 3ʹ-ATG-5ʹ for Met codon AUG, but here for UAC it’s ATG template? No—for AUG mRNA (Met), template 3ʹTAC5ʹ! Let’s fix:
Standard start codon mRNA 5ʹAUG3ʹ, template DNA 3ʹTAC5ʹ, coding 5ʹATG3ʹ.
For anticodon 3ʹAUG5ʹ, pairs mRNA 5ʹUAC3ʹ (Tyr codon).
Template DNA for mRNA 5ʹUAC3ʹ: template 3ʹATG5ʹ (3ʹA (for U), T (for A), G (for C)5ʹ). Yes, so (C) 3ʹ ATG 5ʹ.
Why some sources? PYQ from GATE/CSIR shows options, and logic points C. But earlier thought A for Met, but no—this is unknown aa, for UAC it’s ATG template.
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(A) 3ʹ TAC 5ʹ: Template for mRNA AUG (Met); wrong codon.
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(B) 5ʹ TAG 3ʹ: Coding-like for stop, not matching.
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(C) 3ʹ ATG 5ʹ: Correct template for mRNA 5ʹUAC3ʹ.
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(D) 5ʹ ATG 3ʹ: Coding strand for Met (AUG mRNA), not template.
The anticodon 3ʹ AUG 5ʹ DNA sequence question tests central dogma understanding in molecular biology, vital for CSIR NET Life Sciences.
This MCQ from past papers like GATE Life Sciences emphasizes template strand identification.
Why Anticodon Pairs with Codon
tRNA anticodon 3ʹ-AUG-5ʹ base-pairs antiparallel to mRNA codon 5ʹ-UAC-3ʹ (A-U, U-A, G-C).
DNA Template Strand Derivation
DNA template strand transcribes mRNA: template 3ʹ-ATG-5ʹ → mRNA 5ʹ-UAC-3ʹ (no T in RNA). This matches option (C).
Common Exam Pitfalls
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Confusing template vs. coding strand: Coding is 5ʹ-TAC-3ʹ here.
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Directionality: Options specify 3ʹ-5ʹ or 5ʹ-3ʹ.
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Forgetting RNA uses U, DNA T.
Master anticodon 3ʹ AUG 5ʹ DNA sequence for competitive exams like CSIR NET.


