GATE Biotechnology Previous Year Papers with Solutions Archives - Page 22 of 104 - CSIR NET LIFE SCIENCE COACHING | NTA NET LIFE SCIENCE

The purification data for an enzyme is given below: Step Process Volume (ml) Total protein (mg) Total activity (Units) Specific activity (Units/mg) P Cell-free extract 17 177 102 0.58 Q Q-Sepharose 14 18.8 72 3.83 R Phenyl Sepharose 26 9.2 45 4.89 S Sephacryl S-200 7 4.1 30 7.32 Q.51 The yield (%) for each step is (A) P-10, Q-7.2, R-4.5, S-2.0 (B) P-34, Q-24, R-15, S-1 (C) P-3.4, Q-2.4, R-1.5, S-0.1 (D) P-100, Q-71, R-44, S-29

GATE Biotechnology 2012 Previous Year Papers with Solutions

Enzyme Purification Yield Calculation

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January 18, 2026
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The purification data for an enzyme is given below: Step Process Volume (ml) Total protein (mg) Total activity (Units) Specific activity (Units/mg) P Cell-free extract 17 177 102 0.58 Q […]

GATE Biotechnology 2012 Previous Year Papers with Solutions

Fold Purification Calculation

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January 18, 2026
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The purification data for an enzyme is given below: Step Process Volume (ml) Total protein (mg) Total activity (Units) Specific activity (Units/mg) P Cell-free extract 17 177 102 0.58 Q […]

In a muscle, the extracellular and intracellular concentrations of Na+ are 150 mM and 12 mM, and those of K+ are 2.7 mM and 140 mM, respectively. Assume that the temperature is 25°C and that the membrane potential is −60 mV, with the interior more negatively charged than the exterior. (R = 8.314 J mol−1 K−1; F = 96.45 kJ mol−1 V−1). Q.49 The free energy change for the transport of two K+ into the cell is (A) +8.0 kJ/mol (B) +11.6 kJ/mol (C) +19.6 kJ/mol (D) +31.2 kJ/mol

GATE Biotechnology 2012 Previous Year Papers with Solutions

Free Energy Change for K⁺ Ion Transport in Muscle Cells

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January 18, 2026
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In a muscle, the extracellular and intracellular concentrations of Na+ are 150 mM and 12 mM, and those of K+ are 2.7 mM and 140 mM, respectively. Assume that the […]

GATE Biotechnology 2012 Previous Year Papers with Solutions

Free Energy Change

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January 18, 2026
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In a muscle, the extracellular and intracellular concentrations of Na+ are 150 mM and 12 mM, and those of K+ are 2.7 mM and 140 mM, respectively. Assume that the […]

Q.47 A bacterial culture (200 μl containing 1.8 × 109 cells) was treated with an antibiotic Z (50 μg per ml) for 4 h at 37°C. After this treatment, the culture was divided into two equal aliquots. Set A: 100 μl was plated on Luria agar. Set B: 100 μl was centrifuged, the cell pellet washed and plated on Luria agar. After incubating these two plates for 24 h at 37°C, Set A plate showed no colonies, whereas the Set B plate showed 0.9 × 109 cells. This experiment showed that the antibiotic Z is (A) bacteriostatic (B) bacteriocidal (C) bacteriolytic (D) apoptotic

GATE Biotechnology 2012 Previous Year Papers with Solutions

Unraveling Antibiotic Action

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January 18, 2026
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Q.47 A bacterial culture (200 μl containing 1.8 × 109 cells) was treated with an antibiotic Z (50 μg per ml) for 4 h at 37°C. After this treatment, the […]

Q.46 An enzymatic reaction is described by the following rate expression: v = vmS km + S + S2 / ks Which one of the following curves represents this expression?

GATE Biotechnology 2012 Previous Year Papers with Solutions

Substrate Inhibition Kinetics

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January 18, 2026
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Q.46 An enzymatic reaction is described by the following rate expression: v = vmS km + S + S2 / ks Which one of the following curves represents this […]