Tag: CSIR NET Life Science Previous Year Questions and Solution Genetics

In a transgenic mice line, lox P sites are introduced in the target A in the following manner This transgenic mice line was mated with another transgenic mice line where […]

15. A student was asked to design a knockout cassette for specifically deleting the p53 gene from the prostate gland of mice. Which one of the following pairs of cassettes […]

14. In transgenic mice, the orientation an location of the loxP sites determine whether Cre recombinase induces a deletion, an inversion or a chromosomal translocation. If a researcher wants to […]

13. Which one of the following statements about Cre- mediated site-specific recombination at loxP sites is INCORRECT? (1) When loxP sites flanking a test sequence are oriented in same direction, […]

12. In the following diagram, segments A and C are copies of 10 base pair repeat DNA sequences, flanking a unique stretch shown as B, A and C are in […]

11. The following cassette was designed to create estrogen receptor knock-out mice: SoH: site of homology; Gol: gene of interest What would ensure that proper recombination has taken place? (1) […]

10. A student while constructing knock-out mice isolated mouse embryonic, stem cells and introduced an engineered DNA into the cells. However, none of the mice were transgenic. On checking the […]

9. In an effort to produce gene knockout mice, a gene targeted homologous recombination was tried, with the exogenous. DNA containing neor gene (confer G-418 resistance) and tkHsv gene (confers […]

8. To generate a knock-in or a knock-out mouse, it is essential to use antibiotic selection to select the stem cells that have putatively integrated the construct accurately. Each construct […]

7. Following statements have been made about recombination in a diploid organism: A. Recombination could be identified by genotyping parents and offspring’s for a pair of loci. B. Recombination frequency […]