Q.48 Which of the following is the closest ancestor of all land plants? (A) Blue green algae (B) Red algae (C) Chara (D) Coleochaeteae The correct answer is: (D) Coleochaeteae. In […]
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Which Plant Does Not Use Xylem for Water Transport?
Q.47 Which of the following does NOT use xylem to transport water? (A) Miscanthus (B) Marchantia (C) Selaginella (D) Magnolia The correct answer is: (B) Marchantia. Marchantia is a non‑vascular bryophyte […]
Aleurone Layer of Wheat Seed
Q.46 Which of the following is most abundant in the aleurone layer of wheat seeds? (A) Tannin (B) Starch (C) Protein (D) Lipid The correct answer is: (C) Protein. The aleurone layer […]
Transmembrane Helix Prediction
Q.45 The sequence of a polypeptide that forms a transmembrane helix is shown below. Which one of the following segments of the peptide is most likely to span the membrane? […]
His‑Tagged 40 kDa Protein on Western Blot
Q.44 An N-terminal His-tagged protein of molecular weight 40 kDa was purified using Ni-NTA column. This protein sample was subjected to SDS-PAGE. A western blot of the same using anti-His […]
Net Charge of Polypeptide
Q.43 A polypeptide with the amino acid sequence ‘AGKPDHEKAHL’ was dissolved in a buffer of pH 1.8. The predominant form of the polypeptide will have a net charge of (A) […]
Fumarase Specificity
Q.42 Kinetic parameters for the enzyme fumarase with three different substrates are given below. Substrate […]
Aspartate in Enzyme Active Sites
Q.41 Aspartate residues are found in the active sites of many enzymes. The 𝐩𝐊𝐚 for the 𝜷-carboxylate of aspartate is 3.86 . At physiological pH this group can function as […]
Calculating Fold Purification and Yield
Q.40 An enzyme was purified using ion-exchange chromatography and the results are shown in the table below. Step […]
Restriction Enzyme Digest of Circular Plasmid
Q.39 Three micrograms of a circular plasmid of 4200 bp was digested with a restriction enzyme and subjected to agarose gel electrophoresis. Five DNA fragments of different sizes were observed […]