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87. use of inactivated Sendai virus and PEG for fusion of two cells has proved very beneficial in studies for- (1) Transgenic (2) Tissue culture (3) Physical mapping (4) Recombinant DNA technology

CSIR NET Life Science Previous Year Questions and Solution on GENE MAPPING METHODS

Sendai Virus PEG Cell Fusion Physical Mapping

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December 3, 2025
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87. use of inactivated Sendai virus and PEG for fusion of two cells has proved very beneficial in studies for- (1) Transgenic (2) Tissue culture (3) Physical mapping (4) Recombinant […]

86. The somatic cell hybridization of human and mouse cell can be effectively mediated by (1) CaCl2 treatment (2) Heat Shock (3) Inactivated Sendai virus (4) Enzymatic treatment

CSIR NET Life Science Previous Year Questions and Solution on GENE MAPPING METHODS

Somatic Cell Hybridization Human Mouse Cells

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86. The somatic cell hybridization of human and mouse cell can be effectively mediated by (1) CaCl2 treatment (2) Heat Shock (3) Inactivated Sendai virus (4) Enzymatic treatment Somatic cell […]

85. The following scheme represents deletions (1-4) in the rll locus of phage T4 from a common reference point: (The bars represent the extent of deletion in each case) Four point mutations (a to d) are tested against four deletions for their ability (+) or inability to give wild type (rll+) recombinants. The results are summarized below: a b c d 1 + + + + 2 + + + - 3 + - + - 4 - - + - Based on the above the predicted order of the point mutations is: (1) b-d-a-c (2) d-b-a-c (3) d-b-c-a (4) c-d-a-b

CSIR NET Life Science Previous Year Questions and Solution on GENE MAPPING METHODS

Deletion Mapping of rII Locus in Phage T4

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December 3, 2025
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The following scheme represents deletions (1-4) in the rll locus of phage T4 from a common reference point: (The bars represent the extent of deletion in each case) Four point […]

84. The location of six deletion (Shows as solid line underneath the chromosomes) has been mapped to the Drosophila chromosome as shown in the diagram given below: The recessive mutations a, b, c, d, e f and g are known to be located in the region of deletions, but order of mutations on the chromosome is not known. When flies homozygous for the recessive mutations are crossed with flies homozygous for the deletion, the following result were obtained where the letter 'm' represents a mutant phenotype and ‘+’ represents the wild type. The relative order of seven mutant genes onchromosome is: (1)bceafgd (2) b c d f g e a (3) bc deafg (4) c d e a g f b

CSIR NET Life Science Previous Year Questions and Solution on GENE MAPPING METHODS

Deletion Mapping in Drosophila

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84. The location of six deletion (Shows as solid line underneath the chromosomes) has been mapped to the Drosophila chromosome as shown in the diagram given below: The recessive mutations […]

83. The locations of five overlapping deletions have been mapped to a Drosophila Chromosome as shown below (Horizontal lines in the above figure indicate the deleted regions) Recessive mutations a. b. c, d and e are known to be located within this region, but the order of mutations on the chromosome is not known. When the flies homozygous for the recessive mutations are crossed with flies homozygous for the deletions, the following results are obtained (letter "m" represents mutant phenotype and "+" represents the wild type). On the basis of the above data, the relative order of the five mutant genes on the chromosome is (1) bc de a (2) a b c d e (3) bc e a d (4) c d be a

CSIR NET Life Science Previous Year Questions and Solution on GENE MAPPING METHODS

Deletion mapping in Drosophila

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December 3, 2025
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The locations of five overlapping deletions have been mapped to a Drosophila Chromosome as shown below (Horizontal lines in the above figure indicate the deleted regions) Recessive mutations a. b. […]

82. The following is a schematic representation of region(showing six bands) of the polytene chromosome ofDrosophila, along With the extent of five deletions (Del1 to Del5): Recessive alleles a, b. c. d. e and f are known to correspond to each of the bands (1 to 6), but their order is not known. When the recessive alleles are placed against each of these deletions, the following results are obtained. The plus (+) in the table mdtcates wild type phenotype of the corresponding allele, while a minus indicates the phenotype governed by the corresponding mutant allele. a b c d e f Del 1 + - - - + + Del 2 + + - - + + Del 3 - + - - + + Del 4 - + + - - + Del 5 - + + + - - Which one of the following indicates the correct tocauonof the recessive alleles on the bands of the polytene chromosomes? (1) a-3; b-1; c-2; d-4; e-5; f-6 (2) a-2; b-1; c-3; d-4; e-5; f-6 (3) a-4; b-1; c-2; d-3; e-5; f-6 (4) a-6; b-2; c-3; d-4; e-1; f-5

CSIR NET Life Science Previous Year Questions and Solution on GENE MAPPING METHODS

Deletion Mapping on Drosophila Polytene Chromosomes

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December 3, 2025
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The following is a schematic representation of region(showing six bands) of the polytene chromosome ofDrosophila, along With the extent of five deletions (Del1 to Del5): Recessive alleles a, b. c. […]

81. A novel enzyme was Identified in humans. The followingapproaches are available to identify the chromosome on which the gene encoding the enzyme is present: (A) Suppress the activity of enzyme by RNAi. (B) Identify polymorphism in the population and carry out pedigree analysis to study its inheritance. (C) Purify the enzyme, decipher Its amino acid sequence, predict its DNA sequence and search for its presence in the available human genome sequence. (D) Create chromosome addition lines by making somatic hybrids between human and mouse cells, identify lines showing the enzyme activity and the human chromosome present in it. Which of the above approaches can be used? (1) (A) or (B) (2) (B) or (C) (3) (C) or (D) (4) (A) or (C)

CSIR NET Life Science Previous Year Questions and Solution on GENE MAPPING METHODS

Methods to Identify the Chromosome Location of a Novel Human Gene

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December 3, 2025
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81. A novel enzyme was Identified in humans. The followingapproaches are available to identify the chromosome on which the gene encoding the enzyme is present: (A) Suppress the activity of […]

80 To detect mutation (GAGGTG) allele specific hybridization method is used. Four members of an affected family are investigated. DNA isolated from blood samples of parents and two offsprings are spotted on a membrane after appropriate processing and probed with either TGACTCCTGAGGAGAAGTC (first probe) or TGACTCCTGTGGAGAAGTC (second probe) after labelling. While probed with first oligonucleotide. signals are obtained for the positions where DNA are spotted from parents and offspring II. When probed with second oligonucleotide, signals are obtained at position where DNA from the parents and offspring I are spotted. Results are shown below: Father Mother Off-spring I Off-spring II First Probe + + - + Second Probe + + + - On the basis of the result, which of the following statements is correct? (1) Parents are affected (2) Offspring I is affected (3) Offspring II is carrier (2) Offspring II is affected

CSIR NET Life Science Previous Year Questions and Solution on GENE MAPPING METHODS

Allele-specific hybridization (GAG→GTG) dot-blot

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December 3, 2025
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80 To detect mutation (GAGàGTG) allele specific hybridization method is used. Four members of an affected family are investigated. DNA isolated from blood samples of parents and two offsprings are […]

79.interspecific somatic cell hybrids have proved very useful for (1) Gene mapping (2) Genetic manipulation (3) Gene structure (4) Gene function

CSIR NET Life Science Previous Year Questions and Solution on GENE MAPPING METHODS

Interspecific Somatic Cell Hybrids in Gene Mapping

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December 3, 2025
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79.interspecific somatic cell hybrids have proved very useful for (1) Gene mapping (2) Genetic manipulation (3) Gene structure (4) Gene function Interspecific somatic cell hybrids, formed by fusing somatic cells […]

78 'TaqMan' assay for detection of base substitutions (DNA variants).probes (oligonucleotides) with fluorescent dyes at the 5’-end and quencher at 3’-end are used. While the probe is intact, the proximity of the quencher reduces the fluorescence emitted by reporter dye. If the target sequences (wild type or variant) are present, the probe anneals to the target sequence, downstream to one of the primers used for amplifying DNA sequence flanking the position of variants. For an assay two flanking PCR primers. two probes corresponding to the Wild type and variant allele and labeled with two different reporter dyes and quencher were used. During extension the probe may be cleaved by the Taq-polymerase separating the reporter dye and the quencher. Three individuals were genotyped using the assay. Sample for individual I shows maximum fluorescence for the dye attached to the wild type probe, sample for individual II shows maximum fluorescence for the dye attached to variant probe and sample for individual Ill exhibits equal fluorescence for both the dyes, Which of the following is correct? (1) Individual is homozygous for the variant allele. (2) Individual II is homozygous for the variant allele. (3) Individual is homozygous for the Wild type allele. (4) Individual lit is homozygous for the Wild type allele.

CSIR NET Life Science Previous Year Questions and Solution on GENE MAPPING METHODS

TaqMan Assay Genotyping

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December 3, 2025
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78 ‘TaqMan’ assay for detection of base substitutions (DNA variants).probes (oligonucleotides) with fluorescent dyes at the 5’-end and quencher at 3’-end are used. While the probe is intact, the proximity […]

Tag: CSIR NET Life Science Previous Year Questions and Solution Genetics

Sendai Virus PEG Cell Fusion Physical Mapping

Somatic Cell Hybridization Human Mouse Cells

Deletion Mapping of rII Locus in Phage T4

Deletion Mapping in Drosophila

Deletion mapping in Drosophila

Deletion Mapping on Drosophila Polytene Chromosomes

Methods to Identify the Chromosome Location of a Novel Human Gene

Allele-specific hybridization (GAG→GTG) dot-blot

Interspecific Somatic Cell Hybrids in Gene Mapping

TaqMan Assay Genotyping

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