Technique for Identifying Molecular Markers in Cancer
A patient has been diagnosed with a particular form of cancer. Appropriate treatment of this cancer, however, requires knowledge of which molecular markers are being expressed by the tumor as compared to normal cells of the same tissue. This is most easily accomplished by which of the following techniques?
A. Southern blot
B. Southwestern blot
C.ELISA
D. Microarray analysis
Understanding Molecular Markers in Cancer: Why Microarray Analysis is the Best Choice
When diagnosing and treating cancer, identifying the molecular markers expressed by the tumor is crucial. These markers help determine the tumor’s behavior and response to targeted therapies. Among various molecular biology techniques, microarray analysis is the most effective method for comparing gene expression in cancerous and normal tissue.
Microarray analysis for cancer molecular markers ?
Microarray analysis allows researchers to study thousands of genes simultaneously, identifying which genes are upregulated or downregulated in tumor cells. By comparing gene expression profiles, oncologists can select personalized treatment plans based on the molecular characteristics of the tumor.
Comparison with Other Techniques:
-
Southern Blot (Option A)
- Used for detecting specific DNA sequences.
- Cannot measure gene expression levels.
- More useful for genetic mutations rather than expression profiling.
-
Southwestern Blot (Option B)
- Detects protein-DNA interactions.
- Not relevant for studying gene expression patterns in tumors.
-
ELISA (Option C)
- Used for detecting specific proteins or antibodies.
- Useful for measuring individual biomarkers but not for a broad expression analysis.
-
Microarray Analysis (Option D) – The Best Choice
- Measures the mRNA expression levels of thousands of genes simultaneously.
- Helps identify oncogenes, tumor suppressor genes, and signaling pathways involved in cancer.
- Provides insights into targeted treatment options.
Conclusion:
For determining which molecular markers are expressed by a tumor compared to normal tissue, microarray analysis is the most effective method. It offers a comprehensive gene expression profile, aiding in personalized cancer treatment.
If you’re looking for precise molecular diagnostics in cancer, microarray analysis should be your go-to choice!
54 Comments
Prami Masih
March 4, 2025Okay sir
Aafreen
August 20, 2025Understood by explanation
Southern blotting used for detecting specific DNA sequences. And southern western blotting is used to detect DNA – protein interactions
So correct answer is microarray analysis
Khushi Mehra
August 20, 2025Microarray is a technique in which thousands of genes are studied and measure the mRNA expression level .
Cancer or tumor cells are diagnosed by this method
Varsha Tatla
August 20, 2025Easily clear by explanation
Mahima Sharma
August 20, 2025Micro array. expansion is too 👍🏻 Good
Aman Choudhary
August 20, 2025Option D is correct
Southern blot used for detect specific DNA sequence, Southwestern blot used for detect Dna-protein interaction Elisa used for detect specific Antibody, antigen, protein So microarray is right
Priya choudhary
August 20, 2025Correct answer is option d, because of this southern blotting detect for specific dna,and southwestern used to detect dna for protein interaction so the microarray analysis is correct answer
Sakshi yadav
August 20, 2025Microarray
Deepika Sheoran
August 20, 2025Southern blotting used to detect specific DNA sequence. Southern western blotting is used to detect DNA protein interaction so, microarray is correct.
Neelam Sharma
August 20, 2025Southern blotting
Neelam Sharma
August 20, 2025Microarray
Mitali Saini
August 21, 2025D. Microarray analysis
Sneha .......
August 21, 2025Microarray analysis
Neha
August 21, 2025Microarray analysis measure the mrna expression levels of thousands of gene simultaneously
Khushi Vaishnav
August 21, 2025Microarray analysis is the most effective method for comparing gene expression in cancerous and normal tissue.
Divya rani
August 21, 2025Microarray analysis is the best technique to detect any tumour or cancer cell.
Roopal Sharma
August 22, 2025Microarray assay is best technique for detection of Cancerous cells .
Soniya Shekhawat
August 22, 2025For determining the molecular marker are expressed by tumor compared to normal tissue is by microaaray analysis.
Sneha Kumawat
August 22, 2025Microorray analysis helps identify oncogenes tumor suppressor gens and signaling pathways involve in cancer
Arushi
August 22, 2025Microarray analysis technique is best to obeserve the mrna expression level in cancerous cells
Sonam Saini
August 24, 2025Micro Array Analysis
Kirti agarwal
August 23, 2025Microarry analysis
Priya dhakad
August 23, 2025microarray analysis is the most effective method for comparing gene expression in cancerous and normal tissue.
Priya khandal
August 23, 2025Video se samja ha sir
Akshita Jangid
August 23, 2025Microarray analysis method is used for comparing gene expression in cancerous and normal tissue.
Bharti Yadav
August 23, 2025Microarray is best method for mRNA expression levels in normal cells and cancerous cells.
Ayush
August 24, 2025Microarray analysis checks thousands of genes at once tells which are on or off in cancer vs normal cell
Sheikh Aleen
August 24, 2025Microarray Analysis
Yogita
August 24, 2025Microarray analysis
Himanshi
August 24, 2025Microarray analysis
Reply
Aakanksha Sharma
August 24, 2025Microarray analysis
Shivani
August 24, 2025Microarray analysis is correct answer
AKANKSHA RAJPUT
August 24, 2025Correct answer is option d
Bhavana kankhedia
August 24, 2025Microarray analysis
Kajal
August 24, 2025Micro array analysis measure the expression of thousands of gene simultaneously this is the correct one…
Tanvi Panwar
August 24, 2025Microarray analysis is the best technique to study gene expression levels in tumor cells.
Priyanka Verma
August 24, 2025correct answer is microarray
Parul Yadav
August 24, 2025Best Molecular Biology Technique for identification of Molecular marker is Microarray Analysis.
MOHIT AKHAND
August 24, 2025Done sir ✅
Surendra Doodi
August 24, 2025🤔🤔
Falguni Prakash
August 24, 2025Microarray
Sumit Chhimpa
August 25, 2025Option D is the best option for this ..question because
Microarray Analysis ….measures the mRNA expression levels of thousands of genes simultaneously…. & others technique can’t measures gene expression levels in tumor….
Kajal
August 26, 2025Microarray analysis
Manisha choudhary
August 26, 2025m rna level kaa transcription microarray techniques s hota h sbse phle normal cell and cancer cell kaa dna leneg fir transcription krke m rna s reverse transcriptase enzyme s c DNA bnayenge fir c DNA ko microchip m pore krenge vha phle s hi sample honge
Elisa specific protein or anybody k liye hoti h individual bookmarker hota h
Muskan Yadav
August 26, 2025Microarray analysis is the most effective method for comparing gene expression in cancerous and normal tissue.
Shriyanshi verma
August 30, 2025Easily clear by explanation
Alec
August 30, 2025Microaasay analysis is the most effective for comparing gene expression in cancerous and normal tissues.
Asha Gurzzar
August 30, 2025Microarray is best to detect cancer and tumor
Pratibha Jain
August 30, 2025Correct answer is option (d)
microarray analysis is the most effective method for comparing gene expression in cancerous and normal tissue.
anjani sharma
August 30, 2025Option d
Southern blotting used to detect specific DNA sequence. Southern western blotting is used to detect DNA protein interaction
Anisha jakhar
August 31, 2025Micro array analysis
Shobha Kanwar
August 31, 2025Southern blotting used to detect specific DNA sequence. Southern western blotting is used to detect DNA protein interaction so, microarray is correct.
Neelam Sharma
September 10, 2025For determining which molecular markers are expressed by a tumor compared to normal tissue, microarray analysis is the most effective method. It offers a comprehensive gene expression profile, aiding in personalized cancer treatment.
Deepika sheoran
November 15, 2025Microarrays