The aminoacyl-tRNAsynthetases (AARSS) in an organism have evolved to catalyseaminoacylation of their cognate tRNAs (1) either at the 3′-OH or 2′-OH positions of the adenosine at the CCA end. (2) […]
Tag: DNA REPAIR AND RECOMBINATION PYQ
Tag: DNA REPAIR AND RECOMBINATION PYQ
DNA REPAIR AND RECOMBINATION PYQ
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DNA Repair-Deficient Bacteria Produce More Antibiotic-Resistant Mutants, But Wild-Type Cells Also Generate Resistance
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- June 14, 2025
- 17 Comments
A bacterial culture initiated from a single bacterial cell with a DNA repair- deficient system is inoculated into several individual test tubes and allowed to grow in parallel. Wild type […]
Key Features of the E. coli SOS Response: Role of RecA, LexA, and Translesion Polymerases
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- June 14, 2025
- 12 Comments
The following statements are made about the E.coli SOS response to DNA damage: A. RecA-DNA filament complex stimulates the autoproteolytic activity of the Lex A repressor. B. RecA is activated […]
UV Exposure Is a Primary Inducer of the Bacterial SOS Response: Mechanisms and Implications
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- June 14, 2025
- 14 Comments
Which of the following can induce SOS response in bacteria (1) UV exposure (2) Hydroxyl-amine (3) 5-Fluro-Uracil (4) 2-Aminopurine Which Agents Induce the SOS Response in Bacteria? UV Exposure and […]
Lesion Bypass Prevents Replication Fork Collapse Caused by UV-Induced DNA Damage
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- June 14, 2025
- 13 Comments
UB-induced DNA damage cause advancing replication forks to stall. To avoid a collapse of these stalled replication forks the cell uses: (1) non-homologous end joining (2) lesion bypasss (3) mismatch […]
How TLS DNA Polymerases Reduce Replicative Fidelity by 100-Fold During DNA Damage Bypass
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- June 14, 2025
- 13 Comments
How Translesion Synthesis (TLS) DNA Polymerases Reduce Replicative Fidelity by 100-Fold DNA replication fidelity is critical for genome stability. However, when DNA damage blocks replicative polymerases, cells employ specialized DNA […]
Key Steps of Double-Strand Break Repair (DSBR) Model in Homologous Recombination Explained
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- June 14, 2025
- 2 Comments
Following statements are made about double-strand break repair (DSBR)model of homologous recombination: A. Process of DSBR recombination is triggered by introducing a double-strand break in a DNA duplex B. In […]
RuvB Protein Catalyzes Branch Migration Activity in the Holliday Junction During DNA Recombination
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- June 14, 2025
- 2 Comments
Which one of the following proteins catalyzes branch migration activity in Holliday Junction? (1) Spo11 (2) RuvB (3) Zip1 (4) RuvC Which Protein Catalyzes Branch Migration Activity in the Holliday […]
Roles of E. coli RecA and DnaB Genes: DNA Repair, SOS Response, and DNA Unwinding Explained
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- June 14, 2025
- 11 Comments
In E. coil, recA gene is involved in recombination as well as repair and dnaB gene is involved in unwinding of DNA double strands during replication. Which of the following […]
Recombination Repair Explained: Why Topoisomerase II Does Not Catalyze Homologous Recombination
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- June 14, 2025
- 7 Comments
The homologous genetic recombination is a DNA repair process referred to as recombination repair. Which one of the following statements is INCORRECT for recombination repair? (1) DNA polymerase III stalls […]


