Which one of the following set of essential components are required for Sanger method of DNA sequencing in a required buffer containing MgC12 and Tris-HCl? (1) DNA template, a primer, […]
Tag: DNA Sequencing
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CSIR NET Previous Year Questions on DNA SEQUENCING
South Western blot: Physical interaction between DNA and protein
Which one of the following options represents a combination of terms that are matched INCORRECTLY? (1) ddNTPs : Chain termination ddNTPs: (2) South Western blot: Physical interaction between DNA and […]
CSIR NET Previous Year Questions on DNA SEQUENCING
Nanopore: DNA sequencing SSRS : Co-dominant markers SSRS
Which one of the following combinations of terms is matched INCORRECTLY? (1) Nanopore: DNA sequencing (2) Pyrosequencing : Protein primary structure (3) Homologous recombination : chloroplast transformation (4) SSRS : […]
CSIR NET Previous Year Questions on DNA SEQUENCING
BAC Libraries for Sequencing and Assembling Repeated Regions
Amongst the following. which one is the most appropriate strategy to sequence and assemble highly repeated regions of a genome? (1) Shot gun sequencing (2) Illumina sequencing (3) 454 sequencing […]
CSIR NET Previous Year Questions on DNA SEQUENCING
Pyrosequencing and Emulsion PCR
Which method is used to generate clonally amplified DNA fragments to be used as templates in Pyrosequencing? A. Ligation PCR B. Shot gun cloning C. BAC cloning D. Emulsion PCR […]
CSIR NET Previous Year Questions on DNA SEQUENCING
Codon Bias and Its Relation to Species-Specific Genetic Code Usage
Codon bias related to which of the following? A. Some codons for an amino acids are more frequently used in all species B. Some amino acids are rarely used in […]
CSIR NET Previous Year Questions on DNA SEQUENCING
Effect of High Dideoxynucleotide Concentration in Chain Termination Primer
If the concentration of dideoxynucleotides was too high in a chain termination primer A. The dideoxynucleotides would inhibit the DNA polymerase proceed as the high B. The reactions would yield […]
CSIR NET Previous Year Questions on DNA SEQUENCING
Objective of Performing a Homology Search with a DNA Sequence
What is objective of performing a homology search with a DNA sequence A. To determine if any genes with similar sequences are present in the DNA databases B. To determine […]
CSIR NET Previous Year Questions on DNA SEQUENCING
Human Chromosome Mapping: Importance and Advances
Human chromosomes mapping A. Has been restricted to the sex chromosomes because of small family sizes B. Proceeded much more successfully as large numbers of DNA markers became available C. […]
CSIR NET Previous Year Questions on DNA SEQUENCING
How to Label Oligonucleotides by Polynucleotide Kinase and γ-32P ATP
How can use lable oligo nucleotide (GGATATCC) with any reagents P. [Y-32P] ATP Q. polynucleotide kinase R. DNA-dependent RNA polymerase S. DNA ligase A. P and Q B. R and […]
