Which of these is an advantage of difference in gel electrophoresis (DiGE) compared to gel free approaches (a) More sensitive (b) Intact proteins allow detection of changes in protein modification […]
Tag: SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis)
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CSIR NET Previous Year Questions Gel Electrophoresis
Electrophoresis of Single Stranded DNA or RNA
In case of electrophoresis of single stranded DNA or RNA, which type of gels are used ? (a) Renaturing (b) Denaturing (c) The routine agarose gel (d) The routine polyacrylamide […]
CSIR NET Previous Year Questions Gel Electrophoresis
Amount of agarose added is more Higher agarose concentration
If the amount of agarose added is more, the molecule under analysis should have following characteristic (a) Small size (b) Large size (c) Size has no relation with amount of […]
CSIR NET Previous Year Questions Gel Electrophoresis
How is the size of molecules under analysis measured
How is the size of molecules under analysis measured (a) By measuring the distance moved through a ruler (b) By measuring the amount of visualizing dye used (c) By running […]
CSIR NET Previous Year Questions Gel Electrophoresis
Serum protein separated in agarose gel stained with Ponseau S stain
Serum protein is separated in 1% agarose gel, 25minutes at 100 volts, stained with Ponseau S protein stain. The separation of the protein is not optimal. What would you do […]
CSIR NET Previous Year Questions Gel Electrophoresis
Electroendosmotic flow disturb protein migration in the gel
In electrophoretic separation of proteins in an agarose gel, electroendosmotic flow occurs. This is a buffer flow that may disturb protein migration in the gel. Which of the following alternatives […]
CSIR NET Previous Year Questions Gel Electrophoresis
Total charge of a protein in native gel is determined by the pH
In an electrophoresis system, using a native gel (agarose) proteins are separated due to difference in total charge. What decides the charge of protein ? (a) Voltage (b) Buffer’s pH […]
CSIR NET Previous Year Questions Gel Electrophoresis
2D gel electrophoresis Isoelectric Focusing (IEF) and SDS-PAGE
Which of the following statement about 2D gel electrophoresis is correct? (a) SDS gel electrophoresis and isoelectric focusing both separate native proteins (b) SDS gel electrophoresis and isoelectric focusing both […]
CSIR NET Previous Year Questions Gel Electrophoresis
Isoelectric Focusing-The separation of proteins is only based on charge
Which of the following statement about isoelectric focusing is correct (a) Proteins separated by isoelectric focusing cannot be tested for biological activity (b) Proteins separated by it can be tested […]
CSIR NET Previous Year Questions Gel Electrophoresis
SDS PAGE separate proteins based on their molecular weight
Which of the following statements about SDS PAGE is correct ? a) Wanted proteins can be tested for their biological activity after separation by SDS PAGE (b) Proteins are solubilised […]
