In an electrophoresis system, using a native gel (agarose) proteins are separated due to difference in total charge. What decides the charge of protein ? (a) Voltage (b) Buffer’s pH […]
Tag: Two-Dimensional Polyacrylamide Gel Electrophoresis
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CSIR NET Previous Year Questions Gel Electrophoresis
2D gel electrophoresis Isoelectric Focusing (IEF) and SDS-PAGE
Which of the following statement about 2D gel electrophoresis is correct? (a) SDS gel electrophoresis and isoelectric focusing both separate native proteins (b) SDS gel electrophoresis and isoelectric focusing both […]
CSIR NET Previous Year Questions Gel Electrophoresis
SDS PAGE separate proteins based on their molecular weight
Which of the following statements about SDS PAGE is correct ? a) Wanted proteins can be tested for their biological activity after separation by SDS PAGE (b) Proteins are solubilised […]
CSIR NET Previous Year Questions Gel Electrophoresis
Two dimensional gel electrophoresis is a technique for separating proteins
Two dimensional gel electrophoresis is a technique for separating proteins (A) Based on charge (B) Based on mass (C) Based on both charge and mass (D) Based on its pI […]
CSIR NET Previous Year Questions Gel Electrophoresis
APS (Ammonium persulphate) is used in SDS-PAGE
APS (Ammonium persulphate) is used in SDS-PAGE for (A) preventing oxidation (B) cross-linking (C) its role as a catalyst (D) free radical formation Correct answer: (D) Free radical formation Explanation: […]
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Electrophoresis of a purified protein in SDS-PAGE in the presence of b-marcaptoethanol yields two bands
Electrophoresis of a purified protein in SDS-PAGE in the presence of b-marcaptoethanol yields two bands of 35 kDa and 45 kDa. However, in a gel filtration chromatography, the same protein […]
CSIR NET Previous Year Questions Gel Electrophoresis
What is an issue with using 2D-PAGE
What is an issue with using 2D-PAGE? a. Hydrophobic proteins may not run as expected due to the hydrophobic surfaces. b. Some proteins may not migrate through polyacrylamide and therefore […]
