The tracking dye used in SDS PAGE will be (a) Anionic (b) Cationic (c) Non ionic (d) Amphipathic The correct answer is: (a) Anionic Explanation: In SDS-PAGE, tracking dyes like […]
Tag: Polyacrylamide gel
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CSIR NET Previous Year Questions Gel Electrophoresis
Tracking Dye in SDS PAGE of Protein
Which of these is used as a tracking dye in SDS PAGE of protein (a) Bromo phenol blue (b) Xylene cyanol (c) Orange G (d) All of the above […]
CSIR NET Previous Year Questions Gel Electrophoresis
In Electrophoresis Protein Will Move Until
In electrophoresis, protein will move until (a) Its ph is greater than pi (b) Its ph is smaller than pi (c) Its ph is equal to pi (d) Pi is […]
CSIR NET Previous Year Questions Gel Electrophoresis
Advantage of Difference in Gel Electrophoresis (DiGE)
Which of these is an advantage of difference in gel electrophoresis (DiGE) compared to gel free approaches (a) More sensitive (b) Intact proteins allow detection of changes in protein modification […]
CSIR NET Previous Year Questions Gel Electrophoresis
Electrophoresis of Single Stranded DNA or RNA
In case of electrophoresis of single stranded DNA or RNA, which type of gels are used ? (a) Renaturing (b) Denaturing (c) The routine agarose gel (d) The routine polyacrylamide […]
CSIR NET Previous Year Questions Gel Electrophoresis
The charge present on the DNA backbone is negative
The charge present on the DNA backbone is negative. The force required to accelerate the molecules toward anode is directly proportional to no. of (a) Sugar molecules (b) Phosphate group […]
CSIR NET Previous Year Questions Gel Electrophoresis
Serum protein separated in agarose gel stained with Ponseau S stain
Serum protein is separated in 1% agarose gel, 25minutes at 100 volts, stained with Ponseau S protein stain. The separation of the protein is not optimal. What would you do […]
CSIR NET Previous Year Questions Gel Electrophoresis
Total charge of a protein in native gel is determined by the pH
In an electrophoresis system, using a native gel (agarose) proteins are separated due to difference in total charge. What decides the charge of protein ? (a) Voltage (b) Buffer’s pH […]
CSIR NET Previous Year Questions Gel Electrophoresis
SDS PAGE separate proteins based on their molecular weight
Which of the following statements about SDS PAGE is correct ? a) Wanted proteins can be tested for their biological activity after separation by SDS PAGE (b) Proteins are solubilised […]
CSIR NET Previous Year Questions Gel Electrophoresis
The electrophoresis technique that use isoelectric focusing is
The electrophoresis technique that use isoelectric focusing is (a) AGE (b) PFGE (c) 2D PAGE (d) SDS PAGE Correct answer: (C) 2D PAGE Explanation: Isoelectric focusing (IEF) is a technique […]
